蒺藜苜蓿果刺发育的分子机制研究

项目名称： 蒺藜苜蓿果刺发育的分子机制研究

项目编号： No.31500989

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 何华

作者单位： 中国科学院西双版纳热带植物园

项目金额： 19万元

中文摘要： 种子传播是传播子体离开母株植物散布到新的生境去进行生长繁殖的过程，是当今进化生物学和保护生物学的研究热点之一。种子的传播方式是与植物的生活环境和形态学特征相适应的，如传播体表面具有刺、倒钩或黏液分泌，可通过粘附在动物的皮毛或羽毛上被携带到较远处传播。这种长距离传播对于研究物种的分布和进化具有非常重要的意义。本研究以豆科模式植物蒺藜苜蓿为材料，通过筛选蒺藜苜蓿的Tnt1插入突变体库，分离到三个短果刺突变体short spine(ss)。到现在为止，关于果实果刺发育的分子机制并不清楚。因此我们拟以这三个果刺突变体为突破口，采用分子遗传学的手段，通过插入侧翼序列的连锁分析或图位克隆的方法来克隆影响果刺发育的关键基因。然后通过深入的基因功能研究，找到上述候选基因的互作蛋白以及调控的下游靶基因，最终解析果刺发育的分子机制。

中文关键词： 蒺藜苜蓿；Tnt1插入突变体；果刺；分子机制

英文摘要： Seed dispersal is a process of diaspore moving away from the mother plant to a new environment where they may grow and reproduce, and is a research hotspot for evolutionary and conservation biologists in recent years. The ways of seed dispersal are adapt to the living condition of plants with their morphological characteristics, such as diaspore with a feature of spines, hooks or sticky substances can be carried a long distance to distribute by adhering to the fur or feathers of passing animals. It is very important to study the relationship and molecular mechanism between them. We use Medicago truncatula, a model plant of legume, as our research materials. Three mutants with short spines on its pod surface, a morphological character that can spread seeds by animals far away from the original place, were isolation from the M. truncatula Tnt1 inserting mutant population by the forward genetic screening approach. It is unclear about the molecular mechanism of fruit spine development until now. Hence, we aim to find out the key gene of these three mutants by the linkage analysis of the flanking sequences of the inserting elements and the map-based cloning approaches. Furthermore, with detail function analysis of this gene, we hope to find out the interacting proteins and the target genes of our candidate gene. It will help us to understand the molecular mechanism of the fruit spine development in M. truncatula.

英文关键词： Medicago truncatula; Tnt1 inserting mutants;fruit spine ; molecular mechanism