小麦-黑麦1BL.1RS易位系中反转座子对临近基因表达影响的研究

项目名称： 小麦-黑麦1BL.1RS易位系中反转座子对临近基因表达影响的研究

项目编号： No.31471498

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 唐宗祥

作者单位： 四川农业大学

项目金额： 82万元

中文摘要： 小麦-黑麦1BL.1RS易位系在小麦育种中起着重要作用，而对于1BL.1RS易位染色体与小麦基因组之间的遗传相互作用机制了解甚少。鉴于反转座子是小麦基因组的主要组成部分，且具有重要生物学功能，从反转座子入手进行研究可以丰富这方面的认识。我们获得了1RS来源相同，小麦背景不同的新型初级1BL.1RS易位系。本项目研究将以这些易位系为材料，采用MSAP、cDNA反转座子展示和原位杂交的方法，研究Ty1-copia和Ty3-gypsy类反转座子在1BL.1RS易位系中的转录读出活性及其对临近基因表达的影响，同时了解有转录活性的反转座子在染色体上的分布状况，明确1BL.1RS易位系中，表现转录活性的反转座子家族以及表达受其影响的基因类型，阐明1BL.1RS易位染色体与小麦遗传背景相互作用的分子机制，丰富1BL.1RS用于小麦育种的理论基础。

中文关键词： 小麦；黑麦；1BL.1RS；反转座子；基因表达

英文摘要： Wheat-rye 1BL.1RS translocation lines play an important role in wheat breeding program. The study on the genetic interactions between 1BL.1RS translocation chromosome and wheat genome has been seldom reported. The studying based on retrotransposons will give better understanding of the genetic interactions between 1BL.1RS chromosome and wheat genome because retrotransposons occupy the most component of wheat genome and have important biological function. New primary 1BL.1RS translocation lines, which contain different wheat backgrounds and the same 1RS arms, have been developed. In this project, these 1BL.1RS translocation lines will be used to investigate the transcriptional activity of Ty1-copia-like and Ty3-gypsy-like retrotransposons. In addition, the distribution of the retrotransposons with transcriptional activity on chromosomes and the influence of retrotransposons on the expression of their adjacent genes will be also detected through the methods of MSAP,cDNA-retrotransposon display and in situ hybridization. The aim of this study is to discover the retrotransposon families that can display transcriptional readout activity and the genes that can be influenced by the readout activity in 1BL.1RS translocation lines, and to understand the molecular mechanisms of genetic interactions between 1BL.1RS chromosome and wheat genome. The results of this study will entich the theoretical basis of utilization of 1BL.1RS chromosome in wheat breeding program.

英文关键词： wheat;rye;1BL.1RS;retrotransposon;gene expression