数字基因表达谱及混合模型研究稻瘟病菌细胞自噬与相关通路基因互作

项目名称： 数字基因表达谱及混合模型研究稻瘟病菌细胞自噬与相关通路基因互作

项目编号： No.31300129

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 徐飞

作者单位： 浙江省农业科学院

项目金额： 23万元

中文摘要： 稻瘟病菌的侵染能力与附着胞正常发挥功能呈正相关。附着胞正常发挥作用依赖于胞内具有的巨大膨压，已知这种膨压的产生与细胞自噬过程密不可分。目前稻瘟病菌中的核心自噬基因已基本得到了分离与初步鉴定。但和自噬过程总体研究情况类似，这样的鉴定只限于一些核心蛋白复合物各组分的探讨，而缺乏对于自噬过程整体上的把握以及与该过程相联系的其它信号通路cross-talking情况的了解。高通量测序技术和统计遗传学方法的发展使得对稻瘟病菌自噬过程基因互作状况以及转录水平上的协同调控研究成为现实。本项目拟利用RNA-seq和tag profiling技术获得野生型和△MoATG8突变体在自噬诱导/正常条件下的表达谱，并利用混合模型和生物信息学手段获得差异表达基因之间的互作信息及通路分布情况，初步阐明稻瘟病菌附着胞发育的自噬基因互作网络和转录水平的协同调控机制，寻找自噬过程的新组分。为今后药物靶标筛选提供理论依据。

中文关键词： 细胞自噬；稻瘟菌；基因互作；高通量测序；混合模型

英文摘要： Magnaporthe oryzae is pathogenic to a number of economically important crops such as rice, barley, wheat, and millet. It utilize a specially developed complex infection structure, the appressorium, to gain entry to and colonize plant tissue. The appressorium generates immense intracellular turgor pressure allowing it to penetrate the leaf cuticle. This enormous turgor was a consequence of the accumulation of huge quantities of glycerol in the cell. A number of studies have revealed autophagy is responsible for this process. The core components of the autophagy machinery have been cloned and analyzed. Similar with the progress of autophagy research in other model organisms, such as Saccharomyces cerevisiae and Homo sapiens, the over-all regulatory network of autophagy and the underlay mechanism remained unknown. Until recently, there was a publication described a snap shot of the basal-level autophagy network in H. sapiens. Development of the high-throughput sequencing technology and achievements in statistical genetics made the direct evaluate of gene-gene interaction and transcriptional co-regulation a reality. This program focus on the analysis of autophagy related gene-gene interaction of ΔMoATG8 and the relative widetype under autophagy-induced and normal conditions. By utilizing the powerful mixed-linear mo

英文关键词： Autophagy；Magnaporthe oryzae；Gene interaction；High-throughput sequencing；Mixed model