壳聚糖诱导菜用大豆耐盐性的生理和分子机制研究

项目名称： 壳聚糖诱导菜用大豆耐盐性的生理和分子机制研究

项目编号： No.31260472

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 王聪

作者单位： 内蒙古民族大学

项目金额： 45万元

中文摘要： 壳聚糖对逆境胁迫植物有重要的保护作用。我们的前期工作发现，壳聚糖可降低菜用大豆幼苗的盐害指数、提高其净光合速率及干质量。据此，认为壳聚糖可提高菜用大豆的光合效率，缓解盐胁迫对其造成的伤害。但壳聚糖如何调控菜用大豆光合代谢，进而缓解盐胁迫危害目前尚不清楚。我们将通过叶面喷施壳聚糖溶液的方法，研究盐胁迫下壳聚糖对2个耐性不同的菜用大豆品种的光合特性，叶黄素循环及热耗散，叶绿体抗氧化系统，叶绿素代谢等的影响，明确盐胁迫下壳聚糖保护光合器官，促进色素代谢，提高光合效率，进而提高其耐盐性的生理机制；研究盐胁迫下壳聚糖对菜用大豆叶绿体蛋白质表达及差异蛋白mRNA丰度的影响，阐明差异表达蛋白的生理功能、差异蛋白表达与其mRNA丰度的相关性，揭示壳聚糖诱导菜用大豆耐盐性的分子机制。为外源增施壳聚糖进行菜用大豆抗逆栽培及菜用大豆的遗传改良和抗性育种提供理论依据，获得的差异蛋白质也可为抗性分子育种奠定基础。

中文关键词： 菜用大豆；壳聚糖；光合作用；叶绿体蛋白质组学；

英文摘要： It is known that chitosan plays an important role in protecting biotic and abiotic stressed plants. Our previous work found that chitosan can reduce salt injury index of vegetable soybean seedlings, increase their net photosynthetic rate and dry weight. Accordingly, we believe that chitosan can improve photosynthetic efficiency of vegetable soybean, and then alleviate the injury caused by salt stress. But how the chitosan increases salinity tolerance of vegetable soybean through regulating its photosynthetic metabolism is unclear so far. By foliar application of chitosan, we will study the effects of chitosan on photosynthetic characteristics, xanthophyll cycle and heat dissipation, chloroplast antioxidant system, chlorophyll metabolism of two vegetable soybean cultivars with diferent salt tolerance under salt stress in order to make clear physiological mechanism of chitosan in protecting photosynthetic organ, promoting pigment metebolism, increasing photosynthetic efficiency, and then improving salt tolerance of vegetable soybean. Meanwhile, we will study the effects of chitosan on protein expression of vegetable soybean chloroplast and corresponding mRNA levels under salt stress, and then clarify physiological function of differential proteins and the correlation between the differential protein and mRNA level

英文关键词： vegetable soybean；chitosan；photosynthesis；chloroplast proteomics；