人参皂苷Rg1联合骨髓间充质干细胞对大鼠脑缺血再灌注模型的保护作用及机制的研究

项目名称： 人参皂苷Rg1联合骨髓间充质干细胞对大鼠脑缺血再灌注模型的保护作用及机制的研究

项目编号： No.81202783

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 包翠芬

作者单位： 辽宁医学院

项目金额： 23万元

中文摘要： 骨髓间充质干细胞(BMSCs)移植在防治缺血性脑病及其后遗症方面具有良好的应用前景，但BMSCs移植成活率低、体内存活时间短、移植效果不理想限制了它的应用。人参皂苷Rg1作为BMSCs的诱导剂可以促进BMSCs特化为神经元样细胞，并促进其分化、增殖；同时，Rg1作为小分子物质，易于通过血脑屏障，具有抗炎、抗氧化、清除自由基、改善神经功能缺损、减轻脑梗死体积、抑制细胞凋亡等作用，从而为BMSCs在脑内的存活及分化提供一个良好的微环境，有利于BMSCs在脑内的增殖；因此，有望成为解决这一问题的关键。目前关于人参皂苷Rg1与BMSCs联合应用治疗缺血性脑病的研究还未见报道。本研究通过体外、体内模拟脑缺血动物模型，同时联合应用人参皂苷Rg1及BMSCs进行干预，探讨二者联合应用对于缺血性脑病的保护作用及可能机制，为其作为防治缺血性脑病的新药开发提供实验依据。

中文关键词： 人参皂苷Rg1；骨髓间充质干细胞；脑保护；脑缺血再灌注损伤；凋亡

英文摘要： Bone marrow mesenchymal stem cells ( BMSCs ) transplantation has a good application prospect in the prevention and treatment of ischemic encephalopathy and its sequelae, but the restriction of the use of by the low survival rate of transplanted , short time in vivo survival , transplantation effect is not ideal . Ginseng saponin Rg1 as BMSCs inducer can promote BMSCs differentiated into neuron-like cells, and promote their differentiation, proliferation; At the same time, Rg1 as small molecular substances, easy to pass the blood brain barrier, anti-inflammatory, antioxidant, free radical scavenging, improvement of neurological deficits, reduce the volume of cerebral infarction, inhibiting cell apoptosis, provide a favorable microenvironment for BMSCs in the brain of the survival and differentiation, the benefit of BMSCs in the brain of proliferation; therefore, is expected to become the key to solve this problem. The effect of ginsenoside Rg1 and BMSCs in the treatment of ischemic encephalopathy research has not been reported.This study of simulated cerebral ischemia animal model in vitro and vivo , and combined application of ginsenoside Rg1 and BMSCs intervention, two union application for ischemic encephalopathy protective effect and possible mechanism , provide experimental basis.the prevention and treatm

英文关键词： ginsenoside Rg1；Bone marrow mesenchymal stem cells；brain；cerebral ischemia reperfusion injury；apoptosis