基于物质群指纹特征表达与大蒜辣素有效检测的药用大蒜质量评价研究

项目名称： 基于物质群指纹特征表达与大蒜辣素有效检测的药用大蒜质量评价研究

项目编号： No.21465023

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 关明

作者单位： 新疆师范大学

项目金额： 55万元

中文摘要： 大蒜（Allium sativum L.）为药食两用植物，具有多重功效，被多国药典收载，其质量评价成为目前医药界和药物分析领域的研究热点。本项目拟以大蒜原产地新疆的优势大蒜药材为研究对象，针对不同品种大蒜质量差异明显的现状及大蒜内在质量评价指标- - 大蒜辣素含量检测的瓶颈问题，采用高效薄层色谱技术与化学计量学方法，对其氨基酸类及含硫化合物类物质群的指纹特征进行提取表达，建立具有质量分类评价功能的指纹图谱与特征量化指标；通过筛选替代对照品、确立检测波长，并对样品处理、提取方法和检测方法系统适应性进行探究，建立大蒜辣素超高效液相色谱检测方法。旨在将建立的物质群色谱指纹图谱鉴别与大蒜辣素检测技术应用于新疆特色药用植物大蒜的质量评价、质量控制和新药研发，为新疆地域药材质量评价理论的完善和新型质量鉴别方法的建立提供案例，为提升和完善大蒜药材的质量标准，进一步进行药用大蒜种质资源保护奠定基础。

中文关键词： 大蒜；色谱指纹图谱；大蒜辣素；超高效液相色谱法；质量评价

英文摘要： Garlic has multiple functions and was recorded by pharmacopoeia of many countries, its quality evaluation has become the hotspot in the field of medicine and drug. This project aims to take Xinjiang garlic as the research object, in view of the status of significant differences in quality of garlic and the bottleneck problem of content detection of allicin which was the quality evaluation index, the high performance thin layer chromatography technology and chemometrics methods were used to extract and express the fingerprint characteristics of the material group such as amino acid and sulphur compounds, in order to establish a fingerprint and quantitative feature index which has function of quality classification identification and evaluation,at the same time, through screening of alternative control sample and detection wavelength,exploring the sample treatment, extraction methods and detection methods to establish ultra performance liquid chromatography (UPLC) detection method of the allicin. The objective is to apply chromatographic fingerprint identification of materials group and detection technology of allicin in quality control , new drug research and development of Xinjiang medicinal garlic, to provide the cases for improving the theory on quality evaluation and establishing the new method for quality identification of Xinjiang regional medicinal materials, and to lay the foundation for enhancing and improving the quality standard of garlic, further protection of germplasm resources of garlic.

英文关键词： Garlic;Chromatographic fingerprint;Allicin;Ultra performance liquid chromatography;Quality evaluation