新疆优势资源恰麻古多糖抗肺癌活性及机理研究

项目名称： 新疆优势资源恰麻古多糖抗肺癌活性及机理研究

项目编号： No.81460615

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 海力茜·陶尔大洪

作者单位： 新疆医科大学

项目金额： 47万元

中文摘要： 恰麻古系十字花科植物芜菁 (Brassica rapa L.），有开胸顺气，健胃消食,抗衰老等功效。临床上用于滋阴润肺，治疗肺结核，增强体质。其在维吾尔医药中的应用历史悠久，药用价值显著。我们实验发现：恰麻古多糖含量高达11.53%，其多糖具有较强的抗氧化能力，建立lewis移植性肿瘤模型，进行了恰麻古多糖体内抗肿瘤的初步研究，结果表明：恰麻古多糖高剂量组抑瘤率为55%，多糖与环磷酰胺联合治疗组对肿瘤的抑瘤率为71%。目前用联合柱色谱分离纯化多糖组分，确定了其单糖组成及分子量。鉴于厚实的实验基础，课题组拟研究的内容是：1.采用NMR、UV光谱技术分析和鉴定多糖组分BRP1和BRP2一级结构。2.研究恰麻古多糖组分BRP-1和BRP-2对人肺癌细胞株A549细胞增殖，凋亡诱导，探索肺癌微环境和全身免疫状态与CD4＋、CD25＋、Tr细胞的关系，电镜下观察肿瘤细胞的病理学形态改变状况。

中文关键词： 恰麻古；多糖；抗肺癌；机理

英文摘要： Uyghur drug Qamgur(Latin name: Brassica rapa L. )is the plant from Cruciferae family with effects to arrange gas, stimulate the appetite, alleviate the aging. Qamgur is used Clinically for nourishing Yin and moistening lung, curing tuberculosis and toning up the health system. It's a long history that Qamgur,which has a significant effect and enjoys a high reputation and medicinal value. The study found that content of polysaccharides is high as 11.53% in Qamgur,with powerful anti oxidation effect . Lewis transplanted tumor models were used to study preliminarily on antitumor effect of Qamgur polysaccharides in vivo. And the result showed the tumor inhibition rate of the polysaccharides high dosage group was 55%, and that of the group using polysaccharides combined cyclophosphamide was 71%.so far 7 polysaccharides were got by methods of column chromatography and the monosaccharides and molecular weight had been studied. In view of the basical experimental, the study group intends to study the following: 1. Analysis the chemical structure of polysaccharide fractions by NMR、UV.2. Explore the effect of the homogeneous polysaccharides BRP1 and BRP2 from Qamgur on the proliferation, apoptosis and cycle of human lung cancer cell line A549 and its mechanism. Study the relationship between lung cancer micro-environment , body immune state and the cells CD4＋、CD25＋、Tr. Observe the pathological changes of cancer cell tissues by electron microscope.

英文关键词： BrassicarapaL.;polysaccharides;Anti-lung cancer;Mechanism