磁性纳米微球-细胞亲和毛细管电色谱及其应用研究

项目名称： 磁性纳米微球-细胞亲和毛细管电色谱及其应用研究

项目编号： No.21275169

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 杨丰庆

作者单位： 重庆大学

项目金额： 80万元

中文摘要： 首次以细胞膜色谱和毛细管电色谱理论为基础，将细胞膜色谱发展成为细胞-毛细管电色谱。通过包埋或吸附磁性纳米微球制备细胞固定相，外加磁场为固定力，建立一种具有药物在线筛选及色谱分离分析双重功能的细胞毛细管电色谱法（C-CEC），重点研究巨噬细胞CEC（M-CEC）和细菌CEC（B-CEC）。结合药理学、受体学及色谱理论基础，采用阳性药及阴性药考察所建立方法的适应性、稳定性及特异性等。将M-CEC法应用于人参、黄芪及淫羊藿等中药的分析，并测定准活性成分与巨噬细胞的结合常数（峰漂移法），根据结合常数的大小预测成分的免疫活性。将B-CEC法应用于抗菌药氧氟沙星等对映体的分离分析，并分别测定对映体与细菌的结合常数并预测其抗菌活性。本项目的研究进一步发展了细胞膜色谱法，拓展了毛细管电色谱在药物分析研究领域的应用。

中文关键词： 亲和毛细管电泳；巨噬细胞；血小板；小分子化合物；相互作用

英文摘要： Based on the theroy of cell membrane chromatography (CMC) and capillary electrochromatography (CEC), to develop a cell based CEC. The macrophage ingest or bacteria attached magnetic nanoparticles to prepare the stationary phase, which were fixed in the capillary column by outer applied magnetic field, to develop a cell based CEC (C-CEC) including macrophage CEC (M-CEC) and bacteria CEC (B-CEC), which has both on-line drug screening and chromatographic separation functions. Based on pharmacological, receptor and chromatographic theory,the applicability, stability and specificity of the developed method will be investigated by using positive and negative drug. The M-CEC will be then applied for the analysis of TCMs such as Panax genseng, Radix Astragali and Herba Epimedii. Furthermore, the binding constants of screened out compounds and macrophage will be determined. Based on the binding constants, the immune activities can be predicted. The developed B-CEC method will be applied for the analysis of antibacterial drug such as ofloxacin, and the binding constants of the enantiomers will be determined. Based on the binding constants, the anti-bacterial activities of the enantiomer of ofloxacin can be predicted. In conclusion, the present study will further develop the CMC technique, and brodening the application of

英文关键词： affinity capillary electrophoresis；macrophage；platelet；small molecule compound；interaction