基于谷子杂种优势群的产量性状杂种优势相关基因挖掘及杂优分子机理研究

项目名称： 基于谷子杂种优势群的产量性状杂种优势相关基因挖掘及杂优分子机理研究

项目编号： No.31471563

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 刘正理

作者单位： 唐山师范学院

项目金额： 80万元

中文摘要： 杂种优势群的划分及杂优机理研究已成为当前杂优研究热点，也是解决目前谷子杂交种优势不强，杂交种选育缺乏目的性的根本途径。项目组已综合运用分子、数理和系谱等聚类手段，完成了谷子杂种优势群构建和杂优模式创新工作，为开展杂种优势相关基因挖掘及杂优分子机理研究奠定了基础。本项目计划依据上述结果，配制系列强、中、弱优势杂交种，在孕穗期利用RNA-seq高通量测序技术分析不同类型杂交种与其亲本间的基因差异表达情况；结合它们在单穗重、穗粒重、结实率等产量性状方面的杂种优势特征筛选杂交种与其亲本间的差异表达基因片段；然后通过克隆、测序和生物信息学等手段，挖掘参与产量性状杂种优势形成的相关基因，并明确其功能；通过检测转基因植株和对照中相关基因表达量的变化，初步探明产量性状杂种优势相关基因的表达及调控模式，逐步揭示产量性状杂种优势形成机理，开辟利用杂种优势群研究杂优机理新途径，为培育强优势杂交种提供理论支撑。

中文关键词： 基因表达；杂种优势；基因克隆；功能分析；分子机理

英文摘要： The clustering of heterotic groups of foxtail millet and the research of heterosis mechanism has become a research hotspot, and is the basic solution to solve unsuccessful heterosis utilization and lack of hybrid breeding goal. This project group has finished heterotic group construction and heterosis utilized models innovation of foxtail millet through a combination of molecular, mathematical and pedigree cluster. On this basis, stong heterosis hybrid, middle heterosis hybrid and weak heterosis hybrid will be produced, the high-throughput sequencing of RNA (RNA-Seq) technology was used to analyze gene differential expression between hybrids and their parents at booting stage, and combine with the yield-related traits heterosis characteristics, such as single panicle weight, grain weight per panicle and seed setting rate, to scan differentially expressed gene fragment, and then cloning, sequencing and bioinformatics will be used to mine yield heterosis-related genes and define their functions, preliminary established expression pattern and gene regulation mode of yield heterosis-related genes by examining their expression changes in both transgenic plant and control, gradually revealed the mechanism of heterosis, open up new approaches exploring the mechanism of heterosis using the heterotic group, and provide theoretical support for cultivating stong heterosis hybrid.

英文关键词： Crops;Heterosis;Gene cloning;Functional analysis;Molecular Mechanism