切花菊重要性状分子标记关联分析及相关等位基因挖掘

项目名称： 切花菊重要性状分子标记关联分析及相关等位基因挖掘

项目编号： No.31272196

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 陈发棣

作者单位： 南京农业大学

项目金额： 85万元

中文摘要： 菊花是我国十大传统名花和世界四大切花之一，观赏和经济价值极高。其重要性状多为复杂的数量性状，重要性状相关分子遗传机制研究进展缓慢。为此，本申请项目拟采集切花菊自然群体代表性品种多年多点重要性状表型数据，借助SRAP、SCoT和AFLP等分子标记进行全基因组扫描，采用STRUCTURE 和TASSEL 等软件进行群体结构和连锁不平衡水平分析；发掘与重要性状（观赏性、抗逆性）相关联的分子标记。另一方面，采用候选基因关联分析策略，进行抗逆候选基因的克隆、测序、序列比对，发掘SNPs（或Indel），并用TASSEL软件分析候选基因序列多态性与LD水平及序列多态性与抗逆性的关联，挖掘优异等位基因；采用Arlequin软件进行单体型分析，发掘优异抗逆单体型。本项目的实施可发掘一批重要性状相关联的分子标记、等位基因及优异单体型，初步揭示菊花重要性状的分子遗传机理，为菊花分子设计育种提供理论依据。

中文关键词： 菊花；分子标记；连锁不平衡；关联分析；等位基因

英文摘要： Chrysanthemum is one of the ten traditional important ornamental flowers in China and one of the four most popular cut flowers in the world , chrysanthemum is of high ornamental and economic value. However, the molecular genetic mechanisms of numerous important traits made slow progress because the majority of important traits of chrysanthemum belong to complicated quantity traits. Therefore,in this propasal, a natural population of cut chrysanthemum representative cultivars is employed to testify main morphological and physiological traits at different locations for several years. Genome-wide association strategy will be adopted by means of molecular markers (SRAP, SCoT and AFLP). Population structrue and the extent of linkage disequilibrium in the cut chrysanthemum germplasm will be detected using STUCTURE and TASSEL softwares. Markers significantly related to those important ornamental and abiotic tolerant traits will be discovered. On the other hand, candidate genes association analysis will be conducted as well. Candidate genes related to abiotic stress tolerance will be cloned and sequenced, from which SNPs or Indel will be detected. Diversity of the aligned sequences and the level of linkage disequilibrium will be analyzed, and the associations between nucleotide diversity and abiotic stress tolerance wi

英文关键词： Chrysanthemum；Molecular marker；Linkage disequilibrium；Association mapping；allele