Accurately estimating Origin-Destination (OD) matrices is a topic of increasing interest for efficient transportation network management and sustainable urban planning. Traditionally, travel surveys have supported this process; however, their availability and comprehensiveness can be limited. Moreover, the recent COVID-19 pandemic has triggered unprecedented shifts in mobility patterns, underscoring the urgency of accurate and dynamic mobility data supporting policies and decisions with data-driven evidence. In this study, we tackle these challenges by introducing an innovative pipeline for estimating dynamic OD matrices. The real motivating problem behind this is based on the Trenord railway transportation network in Lombardy, Italy. We apply a novel approach that integrates ticket and subscription sales data with passenger counts obtained from Automated Passenger Counting (APC) systems, making use of the Iterative Proportional Fitting (IPF) algorithm. Our work effectively addresses the complexities posed by incomplete and diverse data sources, showcasing the adaptability of our pipeline across various transportation contexts. Ultimately, this research bridges the gap between available data sources and the escalating need for precise OD matrices. The proposed pipeline fosters a comprehensive grasp of transportation network dynamics, providing a valuable tool for transportation operators, policymakers, and researchers. Indeed, to highlight the potentiality of dynamic OD matrices, we showcase some methods to perform anomaly detection of mobility trends in the network through such matrices and interpret them in light of events that happened in the last months of 2022.
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