ARHGAP9基因在肝癌侵袭转移中的作用及机制研究

项目名称： ARHGAP9基因在肝癌侵袭转移中的作用及机制研究

项目编号： No.81502515

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 张虹

作者单位： 上海中医药大学

项目金额： 18万元

中文摘要： 肝癌是最常见的恶性肿瘤之一，高侵袭转移是其重要的生物学特性，是导致病人死亡的重要原因。尽管有较多的治疗方法，但效果并不理想。我们前期初步研究发现，ARHGAP9基因在肝癌组织中明显低表达，且低表达患者较高表达患者预后差。ARHGAP9高表达还抑制肝癌侵袭转移相关信号通路的激活。本项目拟综合应用生物信息学、细胞分子生物学和数理统计学等多学科知识，采用高通量测序、数据挖掘、Q-PCR、Western blot、基因干扰、基因过表达、免疫共沉淀和激光共聚焦等技术，通过临床大样本检测分析和体内外试验，揭示ARHGAP9基因与临床预后的相关性，阐明该基因对肝癌侵袭转移的影响及机制，探讨其作为肝癌临床预后监测指标和分子治疗靶点的可能性。

中文关键词： 肿瘤；转移；基因；靶点

英文摘要： Liver cancer as one of common malignancies is characterized by high invasion ang migration, which results in high mortality. Although various therapies are employed for liver cancer, the final effect is not very ideal. Our previous studies displayed that the expression of ARHGAP9 was lower in the liver cancer tissue, and the prognosis of low ARHGAP9 expression patients was poor. High expression of ARHGAP9 also inhibited the activation of signalings associated with invasion and migration in liver carcinoma. In this study, we aims to clarify the relevance between ARHGAP9 and the clinical prognosis and to elucidate the effect of this gene on the invasion and metastasis of liver carcinoma by means of high-throughput sequencing, data excavation, Q-RT-PCR, western blot, gene interference, gene overexpression, co-immunoprecipitation, laser confocal microscopy, etc. on the basis of bioinformatics, cell biology and mathematical statistics. This research will provide new sights for understanding the effect and mechanism of ARHGAP9 in invasion, migration and clinical prognosis of liver carcinoma and clarify the possibility of ARHGAP9 to be the clinical prognosis monitoring index and molecular therapeutic target.

英文关键词： Tumor;Metastasis;Gene;Target