PRR11诱导EMT促进胃癌侵袭转移的机制研究

项目名称： PRR11诱导EMT促进胃癌侵袭转移的机制研究

项目编号： No.U1504816

项目类型： 联合基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 胡鸿涛

作者单位： 郑州大学

项目金额： 27万元

中文摘要： 胃癌是我国最常见的消化系统恶性肿瘤之一，其发病率及死亡率居高不下，约60%左右的胃癌患者初诊时已存在淋巴结和/或脏器转移，且转移是影响临床疗效和患者预后的重要因素。我们前期发现PRR11和CTHRC1在胃癌中表达异常，且与淋巴结、远处转移及预后密切相关，敲低PRR11表达，引起CTHRC1表达下降。新近文献报导CTHRC1参与Wnt通路激活，促进肿瘤细胞转移。我们推测PRR11可能通过CTHRC1调控Wnt通路，继而促进胃癌的侵袭及转移。然而，PRR11 调节Wnt通路促进肿瘤转移的文献报道还没有。本研究拟应用组织芯片、基因转染、siRNA干扰、药物阻断以及相关分子生物学技术在组织学、细胞学和功能学水平研究三者的相关性以及调控方式，旨在探讨PRR11在胃癌转移中的作用机制，丰富和完善Wnt通路，为Wnt及其通路在胃癌诊治和转移干预方面提供新的治疗靶点。

中文关键词： C07_胃肿瘤；PRR11；EMT；；转移

英文摘要： Gastric cancer is one of the common malignant tumor in our country，its incidence and mortality significantly higher than that of western countries, About 60% patients with gastric cancer already exists lymph nodes and/or organ metastases, meanwhile metastasis is an independent prognostic factor for gastric cancer. We found respectively PRR11 and CTHRC1 were related with gastric cancer lymph node metastasis and gastric cancer lymph node metastasis were closely related to the prognosis of patients with gastric cancer, low-expressed PRR11 cause CTHRC1 express lower . Recent literature reports CTHRC1 is linked with Wnt and promotes cancer cell invasion and metastasis. we speculate that PRR11 may acting on the Wnt pathway by CTHRC1, and then promote the invasion and metastasis of gastric cancer. However PRR11 regulating Wnt pathway promote cancer cell invasion and metastasis have not been reported. This study intends to application of tissue microarray, gene transfection, siRNA interference, drug block and related molecular biology technology in histology, cytology and function learning level research the correlation and control way, is to investigate the role of PRR11 in gastric cancer transfer mechanism, to enrich and perfect the Wnt pathway in gastric cancer diagnosis and treatment,down to provide new therapeutic target.

英文关键词： gastric tumor;PRR11;epithelial-mesenchymal transition ;transfer