草莓果实中蔗糖应答转录因子及其互作蛋白的分离及功能解析

项目名称： 草莓果实中蔗糖应答转录因子及其互作蛋白的分离及功能解析

项目编号： No.31471851

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 贾文锁

作者单位： 中国农业大学

项目金额： 95万元

中文摘要： 长期以来，蔗糖一直被认为是操纵植物生长和发育的结构和能源物质，而在果树果实发育过程中，糖也一直被认为是与果实品质关系密切的指标。但是，我们最近的研究表明，蔗糖可以作为信号分子在草莓果实发育及成熟过程中起着重要作用。蔗糖操纵草莓果实成熟的重要原因是操纵了ABA信号的产生。本研究旨在以往的工作基础之上，进一步解析蔗糖信号转导与草莓果实发育及成熟调控的关系。研究拟将以蔗糖操纵ABA合成关键酶（FaNCED）基因表达的信号传递链为线索，以FaNCED启动子中蔗糖响应元件的解析为突破口，分离与鉴定蔗糖应答转录因子，并在此基础上，进一步揭示与蔗糖应答转录因子互作的信号蛋白。最后通过代谢组和转录组分析等多种技术与体系，进一步深入解析蔗糖应答转录因子及其互作蛋白在草莓果实发育和成熟中的作用及机理。该研究不仅有助于深入了解草莓果实发育与成熟调控的分子基础，同时对今后果树品质改良的分子调控具有重要的指导意义。

中文关键词： 草莓；蔗糖信号；转录因子；互作蛋白；功能分析

英文摘要： Sucrose has been traditionally thought to be the structural and energy material,and in fruit, sucrose has been traditionally to be the ingredient tightly correlated with fruit quality. However, our recent work demonstrated that sucrose may function as a signal that plays important roles in strawberry fruit development and ripening. The reason for sucrose to regulate strawberry fruit ripening lies in that it may be able to control ABA signal production. Based on previous works, this project aims to further explore the mechanisms for sucrose signal transduction associated with the regulation of strawberry fruit development and ripening. With the signaling pathway of sucrose activated FaNCED expression as a clue, this project will first characterize the sucrose-responsive cis-element in FaNCED promoter. With the cis-element as a bait, we will further identify the sucrose responsive transcription factor, and further more, identify the transcriptor-interacting signal protein. Finally, through metabolic and transcriptional analysis, this project will further decipher the roles of and related mechanisms for the sucrose responsive transcription factor and its interacting protein in the regulation of strawberry fruit development and ripening. This project will not contribute to a better understanding of the molecular basis for strawberry fruit development and ripening, but also be of particular significance for genetic manipulation of fruit quality.

英文关键词： strawberry;sucrose signal;transcrition factor;interacting protein;functional analysis