蓝氏贾第鞭毛虫表面抗原基因转录调控的研究

项目名称： 蓝氏贾第鞭毛虫表面抗原基因转录调控的研究

项目编号： No.30872207

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 李雅杰

作者单位： 大连大学

项目金额： 32万元

中文摘要： 为研究贾第虫表面变异抗原基因vsp基因启动子区的转录元件及转录装置，为探讨其变异机制提供线索。本研究用反向PCR方法克隆了蓝氏贾第虫中国C2株的侧翼序列，含上游2kb和下游1kb序列；进行了5RACE实验，确定了vsp基因的转录起始位点。构建了启动子分析载体和对照载体，转染贾第虫进行了中国株vsp基因启动子活性研究。结果发现，vsp基因转录本具有较短的5UTR区，仅16个核苷酸，5UTR主要为翻译装置识别、结合位点，提示其有独特的翻译起始机制；的核心启动子区存在于0- - -100bp范围内，具体序列有待进一步确定。此外在-100- - -200区域内发现一个负调节元件。负调节元件在真核生物中较少见，可能与vsp变异密切关联，目前正在进一步分析其序列及作用。

中文关键词： 蓝氏贾第虫；表面变异抗原；转录；启动子

英文摘要： To identify transcriptional element and transcriptional machine on the vsp gene promoter of Giardiaand provide clue to discover the mechanisms of giardia antigen variation, the flanking sequence of a Chinese strain ,C2 strain, was cloned by reverse PCR. 2kb upstream sequence and 1kb downstream sequence was obtained. 5 RACE analysis was carried out and the transcriptional start site was determined. This vsp gene contain a short 5UTR sequence and imply a novel translational mechanisms may be involved in the expression of giardia gene. Core promoter may located in -100 region and need further research. A negative regulation element was identified between -100- - -200 region. Its sequence and roles in vsp gene transcriptional regulation remains to be determined.

英文关键词： giardia;vsp;transcript; promoter