一条全新的MPK-WRKY途径调控油菜防御核盘菌的分子机制解析

项目名称： 一条全新的MPK-WRKY途径调控油菜防御核盘菌的分子机制解析

项目编号： No.31301648

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 杨博

作者单位： 西北农林科技大学

项目金额： 25万元

中文摘要： 油菜是我国重要的油料作物之一，核盘菌（Sclerotinia sclerotiorum）引起的菌核病严重危害油菜的产量与品质，研究油菜与核盘菌相互作用分子机制，挖掘其耐受核盘菌侵染的基因并阐明它们作用机制很关键。MAPK与WRKY是植物重要的调控逆境应答的蛋白。我们研究表明核盘菌能调控油菜多个MAPK激酶与WRKY转录因子的表达且wrky tolerant of Sclerotinia (wts1)突变体耐受核盘菌的侵染，还发现一个全新MAPK成员(MAPK1)与WTS1互作，但该MPK1-WTS1调控的抗病反应还未见报道。因此，本项目拟在此研究基础上利用基因沉默、过表达、激酶的组成型活化及靶基因筛选等方法，深入研究WTS1介导的防御信号转导途径及MPK1磷酸化活化WTS1的调控机理，阐明MPK1-WTS1在调控油菜防卫反应中的作用机制，为通过调控关键基因的表达提高油菜抗病性奠定理论基础。

中文关键词： 油菜；WTS1；ROS；Rbohs；衰老

英文摘要： Oilseed rape (Brassica napus L.) is one of the important oilcrops in China. Sclerotinia stem rot caused by Sclerotinia sclerotiorum frequently affects the yielding and quality of oilseed rape seeds. Therefore, it is essential to study and elucidate the physiological and molecular mechanisms of interaction between oilseed rape and S. sclerotiorum as well as to mine those genes that modulate the tolerance against S. sclerotiorum and explore their signal transduction networks. Mitogen-activated protein kinases (MAPKs/MPKs) and WRKY transcription factors are two classes of important proteins that regulate abiotic and biotic stresses in plants. Our previous studies identitified a bunch of MAPK and WRKY genes that were regulated by S. sclerotiorum in oilseed rape through microarray profilings and identified a wrky mutant that is more tolerant of S. sclerotiorum infection and therefore named it wts1 (wrky tolerant of S.sclerotiorum 1). We further identified that MAPK1 interacted with WTS1 protein in a yeast two-hybrid screening. In this proposal, we plan to characterize this MPK-WRKY signaling pathway in order to understand the implicated defense response and molecular mechanims through gene silencing and knock-out, overexpression, and also construction of constitutive active (CA) MAPK1, microarray identification of t

英文关键词： Brassica napus；WTS1；ROS；Rbohs；leaf senescence