一氧化氮荧光探针的构建及其生物细胞成像应用

项目名称： 一氧化氮荧光探针的构建及其生物细胞成像应用

项目编号： No.21302114

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 吕鑫

作者单位： 山西大学

项目金额： 25万元

中文摘要： 一氧化氮是除一氧化碳和硫化氢之外的另一生物气体信使分子，在生命活动中扮演了重要的角色，对一氧化氮的选择性识别与传感在环境监测、疾病诊断等方面具有重要的意义。在各种识别和传感方法中，由于荧光法具有高灵敏度、设备简单、即时监测的优点，因而被化学家和生物学家广泛采用。本项目设计了基于一氧化氮独特化学反应性的荧光探针，拟在一氧化氮传感和生物细胞成像方面展开研究。本项目的创新点主要体现在四个方面：1.设计了基于分子内电荷转移机理以及荧光共振能量转移机理调控的荧光比率一氧化氮探针；2.设计包含有靶向细胞位点的荧光探针，实现细胞器内一氧化氮的定点检测；3. 开发出基于BODIPY荧光团的近红外一氧化氮荧光探针，使其更适合于生物学应用； 4.开发出基于螺环"开-关"原理调控的具有比率性质的近红外荧光染料，并将其应用于一氧化氮荧光探针的设计。

中文关键词： 一氧化氮；荧光探针；生物成像；识别传感；

英文摘要： Nitric oxide is considered as one of the three endogenous gaseous signaling compound (the others are hydrogen sulfide and carbon monoxide).Although the half-life of nitric oxide is only several seconds at low concentrations in biological systems ,it plays important roles in biologicl systems. It is involved in many critical biological processes including signal transduction, smooth muscle relaxation, peristalsis, immune system control, neurotransmission, blood pressure modulation, learning, and memory.However,misregulation of nitric oxide production is implicated with various diseases ranging from stroke, heart disease,hypertension, neurodegeneration, erectile dysfunction, to gastrointestinal distress. Although a wide variety of techniques have been developed to detect nitric oxide, ?uorescence sensing has become the gold standard due to its high sensitivity and simplicity. Thus, the construction of small-molecule ?uorescent probes suitable for speci?c nitric oxide detection in living systems has received great attention.Based on the literatures, we designed series of reaction based nitric oxide fluorescent probes for the imaging of endogenously produced nitric oxide in cells. 1. Based on the intramolecular charge-transfer and fluorescence resonance energy transfer sensing mechanisms,fluorescence ratiometric pr

英文关键词： fluorescent probes；nitric oxide；bioimaging；recognition；