基于长余辉发光纳米探针的前列腺癌细胞表面聚糖原位分析

项目名称： 基于长余辉发光纳米探针的前列腺癌细胞表面聚糖原位分析

项目编号： No.21475106

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 刘英帅

作者单位： 西南大学

项目金额： 85万元

中文摘要： 细胞表面聚糖在细胞生命活动中具有极其重要的作用，研究表明聚糖异常与很多重大疾病如肿瘤密切相关，近年来肿瘤糖生物学日益成为国内外研究的热点。由于聚糖本身的高度复杂性，聚糖的分析检测，尤其是原位成像与解析进展缓慢。申请人在前期研究基础上，创新性地构建一种长余辉发光纳米探针，实现细胞表面聚糖的原位、定量、高灵敏、免激发分析。本项目以不同类型的前列腺癌细胞系为模型，开展以下研究：应用微流控芯片分析正常细胞和不同阶段肿瘤细胞表面聚糖表达和结构的差异；研究抗肿瘤药物对细胞表面糖基表达谱的影响，探讨表面聚糖改变与肿瘤细胞增殖、粘附和迁移的关系；应用抗体微阵列芯片研究前列腺癌细胞表面特定膜糖蛋白的糖基化，从分子水平上了解聚糖异常与前列腺癌的关系及其作用机制。本项目不仅可以为肿瘤细胞表面聚糖的检测和解析提供新的解决途径，促进肿瘤标志物的筛选与肿瘤早期诊断，还可为肿瘤的靶向治疗和药物开发提供理论依据。

中文关键词： 纳米材料；生物传感器；荧光探针；生物芯片

英文摘要： Cell surface glycans play an essential role in various cellular processes including molecular recognition, cell communication, immunoresponse, cell adhesion and migration.It has been demonstrated that aberration of surface glycans is closely related to a lot of fatal diseases such as cancers. In recent years, cancer glycobiology has attracted more and more attentions of worldwide researchers.Since the inherent complexity of glycans, there still remains a great challenge for detection and analysis of cell surface glycans, especially in situ analysis on living cells. Based on the applicant's research experience, this project will innovatively develop a kind of persistent luminescence nanoparticles-based optical probe for in situ, quantitative, highly sensitive, and excitation-free analysis of cancer cell surface glycans. In this project,normal and prostate cancer cell lines are selected as research model. The following subprojects will be investigated in detail: microfluidic lab on a chip is employed to profile glycans on the surface of normal prostate cells and prostate cancer cells; study anti-cancer drugs-induced surface glycan expression and structures changes and further investigate the effect of glycans' aberration on cancer cell proliferation,adhesion,invasion and metastasis; the glycan changes on specific membrane glycoproteins are investigated with antibody microarrays to understand the role of surface glycans in prostate cancer progression and its molecular mechnism.In a word,this project not only provides a novel and powerful technique for in situ imaging and analysis of cell surface glycans, accelerates the screening of cancer biomarkers and early cancer diagnosis, but also renders useful fundemental information for anti-cancer drug discovery and targetted cancer therapy.

英文关键词： Nanomaterials;Biosensor;Fluorescnece probe;Biochip