原位法纳米银海藻纤维的制备及其抗菌机理和细胞毒性作用机制研究

项目名称： 原位法纳米银海藻纤维的制备及其抗菌机理和细胞毒性作用机制研究

项目编号： No.51503110

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 一般工业技术

项目作者： 赵昔慧

作者单位： 青岛大学

项目金额： 20万元

中文摘要： 海藻纤维是一种海洋再生纤维，以海藻纤维为基础进行理论研究，融合纳米技术，开发差别化海藻纤维，对扩展海藻纤维的应用范围，提高其应用价值具有重要意义。为克服直接添加纳米银法中纳米银需要表面改性、改性剂易发泡严重影响纺丝，添加过程难避免纳米银聚集堵塞喷丝孔等弊端，本课题提出一种原位法制备纳米银海藻纤维的新方法。拟通过海藻酸钠基纳米银的合成研究，阐述海藻酸钠在纳米银合成过程中的作用和稳定机理；进而原位法制备纳米银海藻纤维，表征纳米银在纤维中的粒径、分布状况及稳定性，分析纳米银对纤维结构和性能的影响，建立纤维结构与性能的关系；系统研究纳米银海藻纤维的抗菌性能,纳米银在应用过程中的迁移行为，纤维对细菌的细胞壁、细胞膜、DNA、蛋白质等的影响，深入探讨其抗菌机理。通过体外细胞毒性评价，初步探讨其对细胞的毒性作用机制。这些问题的研究对加快开发纳米银海藻纤维及其后期应用具有重要理论指导意义。

中文关键词： 海藻纤维；纳米银；原位法；抗菌性；细胞毒性

英文摘要： The development of differential alginate fiber based on the theory study of alginate fiber and blending with nanotechnology is of importance to enlarge its application field and elevate application value. Silver nanoparticles (AgNPs) are highly effective against bacteria, however, AgNPs tend to aggregate easily, the modifiers of AgNPs are easily produce foam, all these seriously affect the spinning of alginate fiber with AgNPs. Therefore, this program presents a new method of preparing alginate fiber with Ag NPs via in situ method. Firstly, study on the preparation of sodium alginate-AgNPs, the stabile mechanism and interaction of sodium alginate and AgNPs will be elaborate; Then, alginate fiber with Ag NPs will be further prepared via in situ method, Characterization of AgNPs size and distribution in the fibers, Analysis of effect of AgNPs on the structure and properties of fiber, establishing the relationship between structure and properties of the fiber. Study on the antibacterial properties of the prepared fibers and investigation on its antibacterial mechanism via migration behavior of AgNPs in the application process and the effect on the bacterial cell wall, cell membrane, DNA, proteins, et al.. In vitro cytotoxicity evaluation explores possible cell toxicity mechanism. All these research is of great theoretical significance to speeding up the development of alginate fiber with Ag NPs and its later applied.

英文关键词： alginate fiber ;silver nanoparticles ;In situ ;antibacterial property;cytotoxicity