具有取向性内壁的壳聚糖基多孔导管的构建及其调控周围神经再生的机制研究

项目名称： 具有取向性内壁的壳聚糖基多孔导管的构建及其调控周围神经再生的机制研究

项目编号： No.31500782

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 李贵才

作者单位： 南通大学

项目金额： 20万元

中文摘要： 壳聚糖基生物材料广泛应用于周围神经再生领域，通过功能优化改善其对神经细胞生物调控的能力，对增强材料的神经损伤修复功能具有十分关键的科学意义。本项目拟以材料与周围神经再生的界面相互作用为切入点，充分考虑材料表面的拓扑结构及负载生物活性分子对周围神经再生的影响，采用微图形技术结合冻干模塑法及生物分子固定技术，构建表面微图形化的壳聚糖基多孔薄膜和侧壁无需缝合且内壁具有取向性的壳聚糖基多孔导管并负载生物活性分子，体外研究表面微图形及生物活性分子分布对施万细胞生长行为的影响规律，体内评价该神经导管对大鼠坐骨神经损伤修复效果，并阐明材料表面微图形调控神经再生的分子机制，为设计新型神经移植物提供重要理论依据。通过本项目的研究，将在材料表面拓扑结构调控神经再生机制方面取得进一步认识，并建立起构建侧壁无需缝合且内壁具有取向性的天然生物材料多孔神经导管的关键技术，获得具有优异性能的周围神经移植物。

中文关键词： 神经再生；神经导管；内壁取向性；生长因子；拓扑结构

英文摘要： Chitosan-based biomaterials are widely used in the field of peripheral nerve regeneration.It has importantly scientific significance for enhancing the function of repairing nerve injury by regulating nerve cells biofunction via functional optimization. In this study, the interfacial interaction between material and peripheral nerve regeneration is considered as the breakthrough point. The effects of topological structure of the material surface and bioactive molecules on the regeneration of peripheral nerve are fully considered.By combining use of micropatterning technique, freeze-dried moulding method and biological molecular grafting technology, the porous chitosan-based film and conduit with inner wall orientation structure and without suturing side wall grafted with bioactive molecules are constructed. The influence of surface micropatterning and bioactive molecules on the distribution rules and growth behavior of Schwann cells is investigated in vitro in order to obtain the effect of inner oriented and porous chitosan conduit on the repair of rat sciatic nerve injury in vivo, and to study the interaction mechanism between surface micropatterning and Schwann cells behavior, which will provide important theoretical basis for designing new nerve implants. Through the research of this project, the nerve regeneration mechanism regulated by surface topography could be further understood, and the key technique for constructing inner oriented and porous natural biomaterials conduit without sutruing side wall for nerve regeneration could be established, and finally the peripheral nerve grafts with excellent performance are obtained.

英文关键词： Nerve regeneration;Nerve conduit;Inner orientation;Growth Factor;Topography