抗坏血酸缺失介导水稻叶片衰老过程中光合作用“光碳失衡”的机理

项目名称： 抗坏血酸缺失介导水稻叶片衰老过程中光合作用“光碳失衡”的机理

项目编号： No.31270287

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 彭长连

作者单位： 华南师范大学

项目金额： 80万元

中文摘要： 水稻叶片的衰老过程中，光合作用的碳固定能力的下降快于光能吸收能力的下降，导致光能吸收与碳固定的不平衡，吸收的光能不能及时被利用而使活性氧大量迸发，光合酶及光合色素降解，光合功能快速丧失，即"光碳失衡"。本项目利用分子生物学手段获得的抗坏血酸合成关键酶基因GLDH干涉与超表达水稻或拟南芥纯合体为材料，通过分析不同突变体及野生型叶片衰老过程中叶片光能吸收与光合电子的分配、激发压的变化、Rubisco的降解与活性变化、光合色素变化、活性氧代谢及GLDH基因表达等规律，探讨叶片不同抗坏血酸含量与光碳失衡的关系。同时通过在衰老过程中遮光的方式减少叶片光能的获得，或对干涉材料及野生型施加外源的抗坏血酸或刺激内源抗坏血酸合成等来增强清除活性氧的能力等方式，探讨改善叶片衰老过程中光碳失衡的状况。探究防止水稻早衰并增加其结实率和产量的措施，系统阐明水稻叶片衰老的光碳失衡理论，为水稻的高产育种提供理论指导。

中文关键词： 水稻；叶片衰老；光碳失衡；抗坏血酸；活性氧

英文摘要： Carbon assimilation decreases faster than the absorption of light energy in photosynthesis during senescence of rice leaves, resulting in photosynthetic "photon-carbon imbalance". The failure to use the surplus absorbed light energy in carbon assimilation leads to the production of reactive oxygen species (ROS), degradation of photosynthetic enzymes and photosynthetic pigments, and a rapid loss of photosynthetic function, these being consequences of photon-carbon imbalance. As GLDH is a key enzyme in ascorbic acid biosynthesis, this project is designed to study the relationships between ascorbic acid content and photon-carbon imbalance using GLDH RNA interference and overexpression mutants of rice and Arabidopsis as plant materials. The changes of light energy absorption, distribution of photosynthetic electron flow, excitation pressure, degradation and activity of Rubisco, photosynthetic pigments, metabolism of ROS and expression of the GLDH gene will be analyzed during leaf senescense in different mutants and wild- types. Several measures have been designed to improve photon-carbon imbalance such as reduction of absorption light energy by shading, enhancement of the ability for scavenging ROS by feeding exogenous ascorbic acid to the interference materials and wild-types, or stimulation of synthesis of endogen

英文关键词： Rice；Leaf senescence；Photo–carbon imbalance；Ascorbic acid；Reactive oxygen species