redox信号介导的6-BA调控黄瓜弱光适应性的生理与分子机制

项目名称： redox信号介导的6-BA调控黄瓜弱光适应性的生理与分子机制

项目编号： No.31301818

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 姜玉萍

作者单位： 上海市农业科学院

项目金额： 23万元

中文摘要： 弱光是设施蔬菜栽培中的瓶颈问题。氧化还原（redox）信号在植物生长发育和对环境胁迫因子的响应过程中起重要作用，然而其在植物抗弱光胁迫中的状态变化和作用机理尚不清楚。我们初步研究发现6-苄氨基腺嘌呤（6-BA，细胞分裂素的一种）在植物抗弱光胁迫中起调控作用，本项目拟以黄瓜为研究材料，利用化学遗传方法构建不同GSH/GSSG水平的植株，探明6-BA是否通过GSH/GSSG等氧化还原信号元件来调控黄瓜适应弱光环境变化，并利用DIGE荧光差异凝胶电泳（2-D DIGE）,借助Western blot和胶体金免疫电镜确定redox信号调控的蛋白点，进一步推测redox信号在6-BA调控弱光适应性中的作用机理。研究可望阐明弱光条件下redox信号介导的6-BA调控黄瓜弱光适应性的生理与分子机制，为解决设施生产中弱光胁迫的突出问题提供理论依据，具有重要的应用价值。

中文关键词： 6-BA ;；黄瓜 ;；适应性;；弱光;；redox 信号

英文摘要： Weak light is the bottleneck problem in facility vegetable cultivation. Oxidation reduction (redox) signal plays an important role in the process of plant growth, development and the response to environmental stress factors, however, the changes in resistance of plants to weak light stress and the action is unclear. We discovered that 6-Benzylamino adenine (6-BA, a cytokinin) is in regulation of plant resistance to low light stress. The project takes cucumber as study material, using chemical genetic methods to construct different GSH/GSSG standard plants, in order to prove whether the cucumber adapt to weak light stress by 6-BA treatment through redox signal state changes. By using of DIGE fluorescence difference gel electrophoresis (2-D DIGE), with Western blotting and colloidal gold immunoelectron microscopy, the project is aimed at elucidating the protein spots of redox signal regulation. Finally, the project plans to clarify Research is expected to clarify physiological and molecular mechanism of 6-BA in regulating resistance to weak light mediated by redox signaling of cucumber. The project is expected to provide theoretic and practical bases for weak light stress and it has important application value.

英文关键词： 6-Benzylaminopurine ;；cucumber ;；adaptability ;；weak light ;；redox signaling