ROS信号通路与AsA参与棉花纤维细胞发育的作用机制研究

项目名称： ROS信号通路与AsA参与棉花纤维细胞发育的作用机制研究

项目编号： No.30860031

项目类型： 地区科学基金项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 李鸿彬

作者单位： 石河子大学

项目金额： 23万元

中文摘要： 活性氧ROS和抗坏血酸AsA可以通过影响细胞壁疏松及植物激素信号促进细胞伸长发育，并与植株抗逆性紧密相关。2007年，我们首次发现棉花ROS胁迫应答通路的APX基因通过调节乙烯信号和H2O2的平衡参与纤维细胞的伸长发育，并且ROS的水平及与AsA合成密切相关的基因都与纤维伸长发育联系紧密。但是，有关ROS及AsA参与纤维细胞发育过程的调控机制却鲜有报道。本课题通过增强或抑制ROS和AsA的表达水平研究其对纤维发育的影响，分析其对纤维细胞壁结构、组成成分、胞外多糖组成及细胞壁酶类活性的影响；还将研究ROS与AsA的相互作用关系；筛选参与纤维发育的、ROS胁迫通路中调控AsA合成的基因，分析其调节胞内ROS、AsA信号参与纤维发育的作用；最终阐明ROS、AsA、及ROS胁迫应答基因共同参与纤维发育的分子机制。为纤维发育机制的阐明，纤维品质、产量、及植株抗逆性的提高提供理论基础和生产实践参考。

中文关键词： ROS；抗坏血酸；ROS胁迫应答通路；细胞壁；棉花纤维发育

英文摘要： Reactive oxygen species（ROS）and ascorbic acid （AsA）can promote cell elongation through affecting cell wall loosening and plant hormone signal, and is closely related with plant resistance. In 2007, we firstly found that, a cotton APX gene responding to ROS stress participates in promotion of cell elongation by modulating ethylene signal and H2O2 homeostasis, and ROS level and genes closely related with AsA biosynthesis are tightly correlated with the development of cotton fiber cell elongation. However, mechanism of ROS and AsA involved in the regulation of fiber development is rarely reported. The final aim of this project is to elucidate the molecular mechanism of ROS, AsA and genes responding to ROS stress involved in fiber development by studying influence of ROS and AsA to fiber development and doing further research works as followings: analyzing changes of fiber cell wall structure, composition，extracellular polysaccharide and related enzyme activities; investigating the interaction between ROS and AsA; screening genes that respond to ROS stress and regulate AsA biosynthesis during fiber elongating development stage, and analyzing its important role of participant in fiber development by modulating cell ROS level and AsA signal. The above research results will provide a theoretical basis and practice reference for elucidation of fiber development mechanisms, enhancements of yield and quality of fiber, and plant resistance.

英文关键词： ROS;ascorbaic acid;ROS stress-responsive pathway;cell wall;cotton fiber development