玉米渍害胁迫诱导microR528介导的基因表达调控网络的研究

项目名称： 玉米渍害胁迫诱导microR528介导的基因表达调控网络的研究

项目编号： No.31501316

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 刘智捷

作者单位： 华中农业大学

项目金额： 20万元

中文摘要： 玉米是全世界最重要的粮食、饲料和能源作物之一,也是我国第一大粮食作物。随着全球气候的极端变化,涝渍的发生频率在逐年递增,并已逐渐成为继干旱之后威胁玉米生产的第二大非生物逆境胁迫。已有研究证明miRNA在转录和转录后水平上,直接参与发育基因和对非生物逆境胁迫应答基因的表达调控。本研究选用368份关联群体自交系为实验试材料,并选取在冠根特异表达并对渍害逆境响应的miR528作为研究对象。拟建立miR528候选区段与miR528表达以及耐渍表型的关联关系,并筛选酵母单杂交文库,验证参与调控miR528表达的转录因子;利用QPCR和RACE分析并证实渍害胁迫下玉米根系中miR528靶基因的表达,建立miR528介导的基因调控网络并阐明其与渍害胁迫条件下冠根发育间的关系,为玉米耐渍性遗传改良提供新的基因资源和策略。

中文关键词： 水分胁迫；调控网络；基因互作；关联分析；根系

英文摘要： Maize is the one of the world’s most important crop for food, feed and energy. And it is also the largest crop for food in China. As the waterlogging and follding accored more oftern during this decade, waterlogging has become to the second most important abiotic stress in restricting the development of maize production. In eukaryotic genome, there are a large number of non-conding RNAs, especially miRNA, which directly involved in the regulation of the developmental and stress response gene expression in the transcriptional and post-transcriptional level..In this study,the roots from 368 association mapping population were used for analysis. And selected the miR528, which is expecially highly represented in the crown root and in responding to waterlogging stress, as candidate miRNA for research. By association analyzing the miR528 candidate gene with its expression and woterlogging resistant traits in those maize inbred lines. And screening the newly constructed transcription factor library, which invloved in responding to waterlogging stress, to validated the transcription factor binding site on the miR528 promoter. Moreover, qPCR and RACE are used to analyze the miR528 target genes in order to construct miR528 mediated gene regulatory network and characterize the roles of miR528 in responding to waterlogging in maize root cell, which will give a new sight of the miR528 regulation mechanism under waterlogging stress and help on the marker-assisted selection of the waterlogging resistance gene and breed excellent waterlogging.resistance inbred lines.

英文关键词： waterlogging;regulatory network;gene interaction;association analysis;root