奶牛乳腺炎主要病原菌对乳腺纤维化重要细胞生长因子及其受体表达作用的研究

项目名称： 奶牛乳腺炎主要病原菌对乳腺纤维化重要细胞生长因子及其受体表达作用的研究

项目编号： No.31460642

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 王凤龙

作者单位： 内蒙古农业大学

项目金额： 50万元

中文摘要： (限400字)：奶牛乳腺炎的常见病原菌有20多种，但多数地区以金黄色葡萄球菌、链球菌和大肠杆菌感染为主。乳腺炎常引起乳腺组织发生纤维化，并导致奶牛淘汰。本项目在已结题的奶牛乳腺纤维化几种重要细胞因子作用的研究的基础上，拟通过奶牛乳腺炎几种主要病原菌（金黄色葡萄球菌、链球菌、大肠杆菌）对体外培养的奶牛乳腺上皮细胞和成纤维细胞的作用，探讨奶牛乳腺炎主要病原菌对奶牛乳腺上皮细胞和成纤维细胞TGF-β、bFGF和PDGF及其受体表达的作用，以及对奶牛乳腺上皮细胞和成纤维细胞Toll样受体表达和其信号转导途径的影响，以阐明奶牛乳腺炎主要病原菌与乳腺上皮细胞和成纤维细胞纤维化相关细胞生长因子表达、Toll样受体表达和信号转导途径、生长因子受体表达的相互关系，进一步明确奶牛乳腺纤维化的发生发展过程和发病机理，为奶牛乳腺纤维化防治的新方法和新药物研究提供依据。

中文关键词： 奶牛；乳腺纤维化；细胞生长因子；受体；病原菌

英文摘要： (限3000 Characters)：There are more than 20 kinds of common pathogenic bacteria causing bovine mastitis, and Staphylococcus aureus, Streptococcus, and Escherichia coli are major etiologic agents in bovine mastitis in most regions. Bovine mastitis always causes bovine mammary fibrosis, and makes cows eliminated from herd. The project is based on the completed study- - - - The Role of important cytokines in Bovine Mammary fibrosis. We plan to study the effect of the expression of the TGF-β, bFGF and PDGF; the expression of the TβR, bFGFR and PDGFR in bovine mammary epithelial cells and fibroblasts invaded by the main pathogenic bacteria including Staphylococcus aureus, Streptococcus, Escherichia coli in the bovine mastitis in this project, and to study the toll-like receptor expression and its signal transduction pathway in the bovine mammary gland epithelial cells and fibroblasts do by the three kinds of bacteria in vitro, as well as to study the affect of TLRs signaling tranduction pathways for the expression of the three kinds of cell growth factors.Through this project, we will clarify the relation between the main pathogenic bacteria of bovine mastitis and the expression of the several cell growth factor related to mammary gland fibrosis and their receptors . We will clear the role of TLRs signaling pathways for the expression of cell growth factors in bovine mammary gland epithelial cells and fibroblasts invaded by the main pathogenic bacteria causing bovine mastitis. The project further identifies the development process of bovine mammary fibrosis and the pathogenesis. It also provides a foundation for the research of new method and new drug of preventing and controlling bovine mammary fibrosis.

英文关键词： Bovine;the mammary;cell growth factor;receptor;pathogenic bacteria