调控近江牡蛎HSP70基因对污染物响应的转录因子研究

项目名称： 调控近江牡蛎HSP70基因对污染物响应的转录因子研究

项目编号： No.41476100

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 天文学、地球科学

项目作者： 张其中

作者单位： 暨南大学

项目金额： 95万元

中文摘要： 现有研究显示海洋双壳类的HSP70s分子对污染物具有广泛反应性，反应灵敏度高，响应信号持续时间长，是极具潜力的污染预警分子，但温度变化会影响HSP70s表达水平，干扰污染预警准确性。为了解决这一问题，本项目在已经完成的近江牡蛎HSP70基因 5'调控区序列，以及该基因对污染物高表达响应关联位点（遗传标记）的基础上，分离鉴定确认对污染物特异响应的转录因子，研究该转录因子和HSP70分子对热刺激和多种重要海洋环境污染物及其混合物的响应模式，分析两类分子的表达水平及关联性，阐明该转录因子作为污染辅助指示分子消除温度变化对HSP70分子表达水平影响的可行程度，为提高HSP70分子对污染预警的准确性奠定基础；同时，揭示该转录因子在调控HSP70基因对污染物响应中的作用机制。目前还无HSP70基因对污染物特异反应的转录因子的研究报道，所以本项研究不仅具有理论意义，也有显著创新性。

中文关键词： 转录因子；HSP70；污染早期预警；近江牡蛎；海洋环境

英文摘要： Previous studies have illustrated that heat shock protein 70 (HSP70) gene in marine bivalves is in extensive, sensitive, and stable response to various pollutants and their mixture in sea water, then a molecular candidate of early warning biomarker of pollution. However, temperature change can influence expression level of hsp70, and further interferes reliability of warning pollution based on the expression level of hsp70. In order to resolve the issue, in this research project, on the basis of our previous study results of 5'-flaking regulatory region sequence of HSP70 gene in Jinjiang oyster (Crassostrea hongkongensis), and single nucleotide polymorphisms (SNPs) of the regulatory region for high expression of HSP70 in response to various pollutants, the oyster HSP70 transcription factors in unique response to pollutants (not to temperature) will be isolated, purified, and identified. Moreover, the expression patterns of HSP70 and its transcription factors will be further studied in response to heat stimuli and important various pollutants as well as their mixture of marine environment, and be analyzed to evaluate the suitability of the transcription factors taken as supplementary warning molecules of pollution to wipe off the influence of temperature change on expression level of HSP70. This work will lay a strong foundation of HSP70 used as a reliable warning biomarker of pollution, and explore the function and mechanism of the transcription factors in regulation of HSP70 expression in response to pollutants. So far, the HSP70 transcription factors in unique response to pollutants remain unclear, therefore, it is necessary to study the transcription factors, and the research work is full of innovation.

英文关键词： Transcriptional factor;Heat shock protein 70;Early warning molecule of pollution;Crassostrea hongkongensis;Marine environment