拟南芥组蛋白HIS1-3与WRKY1互作调控盐胁迫响应的分子机理

项目名称： 拟南芥组蛋白HIS1-3与WRKY1互作调控盐胁迫响应的分子机理

项目编号： No.31500213

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 樊婷婷

作者单位： 合肥工业大学

项目金额： 20万元

中文摘要： 连接组蛋白H1在染色体重塑和基因表达调控中具有重要的调节作用，然而其在植物响应盐胁迫中的作用尚不清楚。在前期研究中，我们发现HIS1-3基因功能缺失导致植株对盐胁迫耐受，且盐胁迫相关基因SOS1和SOS3的转录水平显著增加，表明HIS1-3可能通过调节SOS1和SOS3转录水平来调控植物盐胁迫响应。此外，酵母双杂技术初步证实HIS1-3与WRKY1之间存在互作，并且WRKY1基因功能缺失的突变体wrky1-1、wrky1-2对盐胁迫表现敏感。生物信息学分析发现SOS1和SOS3的启动子部位含有WRKY转录因子特异性结合的顺式作用元件w-box，暗示WRKY1可能直接调节SOS1和SOS3转录。本项目拟进一步证实HIS1-3与WRKY1互作，研究转录因子WRKY1对靶基因SOS1和SOS3转录的直接调节作用，阐明HIS1-3与WRKY1互作在植物盐胁迫响应中的调控机理。

中文关键词： 盐胁迫；；HIS1-3；WRKY1；SOS1；SOS3

英文摘要： Linker histone H1 has been shown to play an important role in chromatin remodeling and the expression of the genes. However, it is unclear whether H1 is involved in regulating the response of plants to salt stress. In our previous studies, we found that loss-of-function of HIS1-3 gene encoding a structurally divergent histone linker H1 resulted in enhanced salt stress tolerance and increased transcript levels of SOS1 and SOS3 in Arabidopsis, suggesting that HIS1-3 is involved in regulating of salt stress tolerance by affecting the transcript levels of SOS1 and SOS3. Moreover, we preliminarily demonstrated that HIS1-3 interacted with WRKY1 using yeast two hybrid assays, and found that loss-of-function of WRKY1 resulted in enhanced salt stress sensitivity. We also found that the promoters of SOS1 and SOS3 genes contain w-box as the binding site for WRKY transcription factors by bioinformatics anaylsis, implying that SOS1 and SOS3 might be the direct targets of WRKY1. In this work, we will further confirm that the interaction between WRKY1 and HIS1-3, and study the function of WRKY1 in regulating the expression of the downstream genes SOS1 and SOS3, and uncover the molecular mechanisms mediated by the interation between HIS1-3 and WRKY1 in response to salt stress.

英文关键词： salt stress;HIS1-3;WRKY1;SOS1;SOS3