The roto-translation group SE2 has been of active interest in image analysis due to methods that lift the image data to multi-orientation representations defined on this Lie group. This has led to impactful applications of crossing-preserving flows for image de-noising, geodesic tracking, and roto-translation equivariant deep learning. In this paper, we develop a computational framework for optimal transportation over Lie groups, with a special focus on SE2. We make several theoretical contributions (generalizable to matrix Lie groups) such as the non-optimality of group actions as transport maps, invariance and equivariance of optimal transport, and the quality of the entropic-regularized optimal transport plan using geodesic distance approximations. We develop a Sinkhorn like algorithm that can be efficiently implemented using fast and accurate distance approximations of the Lie group and GPU-friendly group convolutions. We report valuable advancements in the experiments on 1) image barycenters, 2) interpolation of planar orientation fields, and 3) Wasserstein gradient flows on SE2. We observe that our framework of lifting images to SE2 and optimal transport with left-invariant anisotropic metrics leads to equivariant transport along dominant contours and salient line structures in the image. This yields sharper and more meaningful interpolations compared to their counterparts on $\mathbb{R}^2$
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