S100A6基因修饰的骨髓间充质干细胞移植治疗糖尿病性勃起功能障碍的机制研究

项目名称： S100A6基因修饰的骨髓间充质干细胞移植治疗糖尿病性勃起功能障碍的机制研究

项目编号： No.81471450

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 张滨

作者单位： 中山大学

项目金额： 67万元

中文摘要： 糖尿病性勃起功能障碍（DED）的发生和发展与阴茎海绵体内皮细胞（CCECs）功能损害有着密切关系。因此研究CCECs的损伤机制、针对CCECs功能的损伤进行保护和修复，是当前的研究热点。我们前期采用蛋白质组学方法从源头筛选出了与CCECs受损密切相关的因子（S100A6）。发现S100A6在DED大鼠海绵体组织中表达明显下调；过表达S100A6能显著改善CCECs的生长状态及功能，以及DED大鼠的勃起功能。但是有关S100A6在CCECs内的作用机制尚不清楚。因此，本研究将在体外实验和动物模型中进一步阐述S100A6在CCECs中的作用及其详细调控机制；并利用S100A6基因修饰具有组织修复功能的骨髓间充质干细胞，探索和验证其作为DED干细胞治疗新手段的可行性与疗效，为DED治疗提供新的措施和思路。本研究对于阐明DED的发病机制及提高其治疗水平具有重要的理论价值和现实意义。

中文关键词： 勃起功能障碍；糖尿病；S100A6；骨髓间充质干细胞；海绵体内皮细胞

英文摘要： Reduced function of the corpus cavernosum endothelial cells (CCECs) is closely associated with the progression of diabetic erectile dysfunction (DED). To study what mechanism is responsible for the injury of CCECs and how to protect and reverse the function of CCECs from the injury have become to the hot research field now. Our previously proteoimcs study has found that S100A6, a novel factor may play an important role in CCECs injury, was significantly decreased in the corpus cavernosum tisse of DED rat. Overexpression of S100A6 in vitro and in vivo can significantly improve the viability and activity of CCECs, and erectile ability of rat, but the mechanism underlying these processes remains obscure. In this study, we plan to elucidate the potential regulatory role of S100A6 in CCECs. Furthermore, this study is designed to investigate the effect of S100A6 adenovirus-transfected bone marrow mesenchymal stem cells (BM-MSCs) on improving erectile function in diabetic rats, and to assess the feasibility of combinating stem cells and gene transfection as a new stratagy in treating of DED. We suggested that this project is value to us improving our understanding of DED.

英文关键词： erectile dysfunction;diabetes;S100A6;BM-MSCs;corpus cavernosum endothelial cells