脂联素特异性调节的miRNA在糖尿病肾病中的作用及机制研究

项目名称： 脂联素特异性调节的miRNA在糖尿病肾病中的作用及机制研究

项目编号： No.81460162

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 焦谊

作者单位： 新疆医科大学

项目金额： 48万元

中文摘要： 糖尿病肾病（DN）是糖尿病最常见的并发症之一，是慢性肾衰的常见病因，其发病机制尚不清楚。miRNA作为一种全新的调控机制广泛的参与了DN发生和发展，DN时肾脏内一系列的miRNA激活并形成复杂的调控网络，研究miRNA与DN的关系，将为理解DN发病及治疗提供新的靶点。脂联素（APN）是目前发现的具有抗炎、改善胰岛素抵抗的重要脂肪细胞因子，其可以通过调节miRNA的表达而发挥抗炎的作用，同时临床研究表明血浆APN浓度与DN关系密切。但脂联素是否通过特异性调节microRNA来实现对DN的保护作用目前尚未见报道。本研究拟用miRNA-seq技术筛选出DN小鼠肾脏差异表达的miRNAs后，在DN小鼠肾脏过表达APN来寻找其特异调节的miRNA，并分析它们在DN中的作用机制。研究脂联素特异性调节的microRNA极有可能为治疗DN提供更加丰富的靶点，并为抗DN药物家族添加更多新药成员。

中文关键词： 脂联素；微小RNA；糖尿病肾病

英文摘要： Diabetic nephropathy (DN) is the most common complication of diabetes , which is a common cause of chronic renal failure ,but the pathogenesis is unclear. As a new regulatory mechanism, miRNA involved in a wide range of development and progression of DN , miRNA actived and formed a series of complex regulatory networks in DN. The study of the relationship between miRNA and DN will provide new targets for understanding the pathogenesis and treatment of DN. Adiponectin (APN) has acted as a major adipokines of the anti-inflammatory and improving insulin resistance, which can modulate the expression of miRNA in the role of anti-inflammation. At the same time , clinical studies have shown the close relationship between the plasma APN and DN.But whether adiponectin by specific regulation of microRNA to achieve the protective effect of DN has not been reported . This study intends to use miRNA-seq technology to filter out different miRNAs of DN in mouse kidney, later to find the specific miRNA regulated by overexpressing APN, and to analyze their role in DN. Adiponectin specific microRNA regulation is likely to provide a richer target for the treatment of DN, and add more anti-DN drug family members .

英文关键词： adiponectin;microRNA;Diabetic Nephropathy