基于“脑-肠互动”研究溃疡性结肠炎脑、肠微环境中IL-6/IL-6R 信号通路的响应及疏肝健脾法的干预作用

项目名称： 基于“脑-肠互动”研究溃疡性结肠炎脑、肠微环境中IL-6/IL-6R 信号通路的响应及疏肝健脾法的干预作用

项目编号： No.81460696

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 朱向东

作者单位： 甘肃中医药大学

项目金额： 50万元

中文摘要： 溃疡性结肠炎（UC）是严重危害人体健康的疑难肠病，精神心理的恶化是其反复发作的重要原因。我们前期研究发现，以疏肝健脾法为指导的痛泻要方对肠黏膜炎症损伤具有明显保护作用；临床观察痛泻要方不仅能改善UC患者的躯体症状，且具缓解其精神情绪的可靠临床疗效，但其作用机理不明。研究发现而炎症反应因子IL-6可作为一种新型的脑肠肽作用于中枢进而影响肠道炎症的形成。故研究从脑-肠互动途径建立疏肝健脾法治疗UC的作用机制可能与干预中枢IL-6及其受体的信号通路密切相关的假说，采用TNBS/乙醇结合束缚应激法建立肝郁脾虚UC大鼠模型，以痛泻要方进行干预，采用ELISA、免疫荧光、免疫印迹、RT-PCR等技术检测脑、肠组织中IL-6及受体信号转导相关因子的基因和蛋白表达，拟证实疏肝健脾法治疗UC的疗效机制与调控中枢神经系统异常相关，揭示疏肝健脾法治疗UC的新靶点，为开发防治UC的优势中成药提供理论支撑。

中文关键词： 脑-肠互动；溃疡性结肠炎；IL-6/IL-6R；信号通路；疏肝健脾法

英文摘要： Ulcerative colitis (UC) is a kind of difficult enteropathy disease, serious harm to human health, and psychological deterioration is the important reason for their repeated attacks. Under the Shuganjianpi treatment, Tong Xie Yao Fang can not only improve the physical symptoms of patients with UC, numerate ease the mental or emotional reliable clinical efficacy, but its mechanism of action is unknown. Our previous study found, the Tong Xie Yao Fang has protective effects of TNBS / ethanol-induced rat intestinal mucosa harm. Research shows that the formation of inflammatory factor IL-6 can be used as a new brain gut peptide interaction model in the central and the effect of intestinal inflammation.Combines the advantage of Chinese medicine treatment of UC and preliminary basis, establish Shuganjianpi treatment healing mechanism of UC may be closely related to the intervention central IL-6 and its receptor signaling pathway hypothesis from the brain - gut interaction; use TNBS / ethanol combined restraint stress to build the Stagnation and Spleen deficiency UC rat model intervene by representative Tongxieyaofang of the Shuganjianpi treatment, using ELISA, immunofluorescence, Western blot, RT-PCR to detect colon and brain tissue of IL-6 and its receptor, IL-6 receptor signal transduction related factor gene and protein expression, to be confirmed Shuganjianpi treatment of UC efficacy mechanism and regulation of the central nervous system abnormalities closely related, the reveal Shuganjianpi treatment as a new target for the treatment of UC is to provide theoretical support for the development of prevention UC advantage of proprietary Chinese medicines.

英文关键词： Cerebral brain - gut interaction;Ulcerative Colitis;IL-6/ IL-6Rsignaling pathway;Shuganjianpi treatment