棉铃虫神经肽促咽侧体素GPCR的鉴定及新型配体筛选

项目名称： 棉铃虫神经肽促咽侧体素GPCR的鉴定及新型配体筛选

项目编号： No.31272111

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 魏兆军

作者单位： 合肥工业大学

项目金额： 80万元

中文摘要： 昆虫神经肽及其G蛋白偶联受体(GPCR) 在调控其生长、发育、生殖、神经传导、代谢以及行为等方面起重要的作用，是绿色农药设计重要的靶基因。昆虫的促咽侧体素(AT)具有促进JH合成等重要功能。本研究拟克隆棉铃虫神经肽促咽侧体素(Har-AT)的G蛋白偶联受体(Har-ATR)基因的全长序列，基于细胞模型明确其相应配体，明确棉铃虫AT与ATR作用的第2信使；设计和合成不同模拟AT结构的小分子化合物，分析其对棉铃虫ATR的激活活性或拮抗作用，筛选出棉铃虫AT的GPCR的高效结合的新型配体；采取直接注射或体外培育的方法，进一步调查高效结合的新型配体的生理作用，筛选出具有生理功能的人工新型配体。本研究为进一步把这些新型配体开发成控制棉铃虫生长发育的绿色农药建立基础；建立的G蛋白偶联受体研究平台，可以用来筛选昆虫其他神经肽受体的激活剂或拮抗剂，为高通量筛选绿色农药、控制害虫的危害建立基础。

中文关键词： 棉铃虫；促咽侧体素；G蛋白偶联受体；配体；信号通路

英文摘要： Neuropeptides and their G-protein coupled receptors (GPCRs) play key roles in regulation of insect growth, development, reproduction, nervous transduction, metabolism, behavior and other aspect of life, which can be used as a target model to develop novel agents for pest control. Insect allatotropin can stimulates JH synthesis in the corpora allata, which can regulate insect development. In the present research, the full length of cDNA of the gene enconding allatotropin G-protein coupled receptor in Helicoverpa armigera (Har-ATR) will be cloned and sequenced. Based on the cell model, the binding activity of ATs to ATR will be detected, and the best ligand to Har-ATR will be decided. The second message of signal pathway of AT to ATR will be illuminated in Helicoverpa armigera. The mimetic peptides or chemicals will be designed and synthesized based on the ligands to Har-ATR, which will be used to detect the ability of agonist or antagonist to Har-ATR. If showing high ability of agonist or antagonist, the mimetics will be selected as the candidate artificial ligands. The effects of candidate artificial ligands to insect will be clarified using injection of insect or in vitro assay. And then, the ligands those showed high effects will be selected as novel agents for pest control. The results of the present research

英文关键词： Helicoverpa armigera；Allatotropin；G protein coupled receptor；Ligand；signal transduction