级联信号放大疾病标志物高灵敏、多组分同时电化学生物传感研究

项目名称： 级联信号放大疾病标志物高灵敏、多组分同时电化学生物传感研究

项目编号： No.21275004

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 向云

作者单位： 西南大学

项目金额： 80万元

中文摘要： 围绕青年基金项目研究主题，我们根据鸟嘌呤的电化学响应以及酶、量子点为标记，实现了尿路感染病原体免标记、高灵敏电化学检测。通过研究我们还建立了同一电极界面多组分生物分子的同时电化学传感及基于双重分析信号放大的高灵敏生物分子电化学检测技术。研究成果已发表SCI 论文17 篇，其中IF>5.0的论文13篇(Anal. Chem. 2篇，Chem. Commun. 5篇，Chem. Eur. J. 1篇， Biosens. Bioelectron. 5篇)。基于青年基金研究的良好进展及基础，在此我们申请青年-面上连续资助。申请项目拟以前列腺癌标志物，肝癌三种联合检测标志物及三种病毒的DNA标志物为研究对象，结合生物条形码信号放大技术与酶辅助氧化还原循环、目标DNA循环及量子点信号增强功能，建立级联分析信号放大策略，实现标志物超灵敏、多组分同时电化学检测。该项目是青年基金研究工作的进一步深化与拓展。

中文关键词： 电化学生物传感；信号放大；高灵敏；标志物；

英文摘要： Under the support of the National Natural Science Foundation of China (NSFC) for young investigators, we have achieved highly sensitive detection of uropathogens based on enzyme and quantum dot labels. We also developed label-free, sensitive strategies for electrochemical detection of uropathogens. At the same time, by using labels with distinct electrochemical signatures, we have developed methods for one-spot, simultaneous detection of different biomolecular targets and proposed several dual signal amplification approaches for highly sensitive electrochemical detections via enzyme-assisted redox-recycling. These research results were published in some of the high impact peer-reviewed journals, including 2 in Anal. Chem., 5 in Chem. Commun., 1 in Chem. Eur. J. and 5 in Biosens. Bioelectron. Based on the excellent progress on the NSFC funded project, here we apply for continuous support from the NSFC to develop new cascade signal amplification strategies for ultrasensitive and simultaneous detection of protein biomarkers for various cancers and DNA biomarkers for different virus. The cascade signal amplification will be achieved through the integration of the biobarcode signal enhancement with enzyme-assisted redox-recycling, target DNA recycling as well as quantum dot-labeled amplification. The proposed study i

英文关键词： Electrochemical biosensors；Signal amplification；High sensitivity；Biomarkers；