填埋场中甲烷-Fe(III)/ Fe(II)-硝氮交互作用关系及其微生物分子生态机理研究

项目名称： 填埋场中甲烷-Fe(III)/ Fe(II)-硝氮交互作用关系及其微生物分子生态机理研究

项目编号： No.51478432

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 其他

项目作者： 沈东升

作者单位： 浙江工商大学

项目金额： 85万元

中文摘要： 本项目以生物反应器填埋场为载体，首先通过稳定同位素示踪、铁元素形态分析并结合电子流向分配计算，揭示生活垃圾填埋场中甲烷、Fe(Ⅲ)/Fe(Ⅱ)和硝态氮之间的交互作用关系；其次，采用高通量测序、计算机检索并结合纯化培养分析，探明生活垃圾填埋场中参与硝酸型甲烷氧化（NAOM）、铁还原型甲烷氧化（FeAOM）和亚铁氧化型反硝化（FeADN）过程的主要微生物菌群；最终，利用功能菌群信号分子释放、外源信号分子添加并结合群体感应淬灭，分析群体感应对物质代谢-种群动态、物质代谢-功能基因表达的影响，探究群体感应对生活垃圾填埋场中甲烷、Fe(Ⅲ)/Fe(Ⅱ)和硝态氮三者交互作用过程的生态调控机理。项目的预期研究成果将丰富源头同步削减填埋场甲烷和氮素排放的手段，从物质代谢、微生物生态、功能基因表达调控多层面揭示填埋场污染物的自净机理。

中文关键词： 填埋场；甲烷减排；脱氮；铁；群体感应

英文摘要： Based on the bioreactor landfill, this research will reveal the interrelationship among methane, Fe(III)/Fe(II) and nitrate by using isotopic tracing, iron speciation analyses and electronic flow distribution calculation firstly. Subsequently, the main strains of nitrite-driven anaerobic methane oxidation (NAOM), iron-mediated anaerobic methane oxidation( FeAOM) and iron-supported autotropic denitritication (FeADN) will be explored by using high-throughput sequencing, computer indexing and isolation together. Finally, the effect of quorum sensing on substance metabolism-community dynamics and substance metabolism-functional gene expression could be clarified by analyzing the release of auto-inducers by the functional bacteria, quorum sensing induced by exogenous auto inducers and quorum quenching by quenching reagent. Moreover, effect of quorum sensing on the ecological controlling mechanism among methane, Fe(III)/Fe(II) and nitrate will also be elucidated. Results of this research will enrich the corresponding source reduction of methane and nitrogen technologies and methods, and also can elucidate the pollutant self-attenuation mechanism from metabolism, microbiological, and functional gene mediation together.

英文关键词： Landfill;Methane reduction;Denitrification;Iron;Quorum sensing