氧化石墨烯强化火凤凰修复PAHs-Cd复合污染土壤及影响机制

项目名称： 氧化石墨烯强化火凤凰修复PAHs-Cd复合污染土壤及影响机制

项目编号： No.31470547

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 刘睿

作者单位： 中国科学院沈阳应用生态研究所

项目金额： 90万元

中文摘要： 申请项目针对辽宁省PAH-Cd复合污染典型土壤，选用本项目组多年筛选出有较好修复效果的植物火凤凰，利用纳米材料氧化石墨烯研究强化火凤凰修复PAH-Cd复合污染土壤效能，并从植物根际土壤和植物体两大方面，深入研究植物修复作用机制。一方面分析根际土壤中酶系、微生物群落结构、根际分泌物三者之间与PAHs、Cd降解及PAHs代谢产物的响应作用关系；另一方面分析植物体内影响其抗氧化防御系统的两大重要酶系与污染物降解的响应关系，探讨植物体内酶系在植物修复过程中调控作用。同时，运用与拟南芥基因芯片杂交，研究在氧化石墨烯强化植物修复中，影响火凤凰降解PAHs和Cd的基因差异分析，并通过iTRAQShotgun蛋白组学研究，分析火凤凰降解PAHs和Cd的蛋白组学应答机制。本项目申请是探求一条更有效解决PAHs-Cd复合污染土壤治理的新途径，同时为更好的深入研究复合污染土壤治理及其机理研究提供更有力的科学依据

中文关键词： PAHs-Cd复合污染土壤；氧化石墨烯；酶系；微生物群落；基因及蛋白组学

英文摘要： According the condition of combined pollution soils in Liaoning region, pot experiment was performed to research the effectiveness and mechanisms of phytoremediation in PAHs-Cd contaminated soils after strengthening by grapheme oxide, based on the plant screened (Fire Phoenix), which have high effectiveness of removal rates for PAHs and Cd. The mechanism of phytoremediation was deeply studied in the two aspects of rhizosphere soil and plant. The relation of enzyme system, microbial communities structure and root exudate in rhizosphere soil with PAHs, Cd degradation and PAHs metabolites was analyzed on one hand; The response relation of two major enzyme systems in the plant affecting the antioxidant defense system with pollutant degrading was analyzed, and the control function of enzyme system in the plant during phytoremediation was discussed on the other hand. Meanwhile, differential expression of gene affecting degradation was analyzed during phytoremediation strengthened by graphene oxide. The proteomics response mechanism was studied through iTRAQShotgun. It may provide a new approach to solve PAHs-Cd combined pollution soil for phytoremediation. And more powerful scientific basis was provided for improving the control and mechanisms in combined pollution soils.

英文关键词： PAHs-Cd combined pollution soils;graphene oxide;enzyme system;microbial communities;gene and proteomics response