骨髓间充质干细胞移植对胶原诱导关节炎大鼠作用机制的研究

项目名称： 骨髓间充质干细胞移植对胶原诱导关节炎大鼠作用机制的研究

项目编号： No.81202356

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学免疫学、法医学

项目作者： 高晋芳

作者单位： 山西医科大学

项目金额： 23万元

中文摘要： 类风湿关节炎(RA)是一种高度致残性的自身免疫性疾病，目前早期联合使用改善病情的抗风湿药物治疗RA已经达成共识，但是这些联合方案的临床缓解率仅为40%-70%。而病情发展出现关节畸形时，尚无治疗方法能够修复这种软骨及骨的破坏。所以寻找新的有效治疗RA的方法是目前国内外风湿病学界致力研究的热点。间充质干细胞（MSCs）是一种具有高度自我增殖能力和多向分化潜能的干细胞。最近研究发现其具有免疫调节作用。我们的前期研究已证实MSCs移植可以改善胶原诱导关节炎（CIA）大鼠的关节症状。本课题以CIA大鼠为研究对象，分早期治疗组和晚期治疗组经大鼠尾静脉输注MSCs，从移植大鼠的免疫细胞和免疫分子等多角度探讨其免疫调节作用的机制；从MSCs体内迁移、定植、分化多方面探讨其修复关节软骨和骨的作用机制。为临床利用MSCs移植治疗RA提供实验基础和理论依据。

中文关键词： 间充质干细胞；类风湿关节炎；绿色荧光蛋白；免疫调节；分化

英文摘要： Rheumatoid arthritis (RA) is a highly disabling of autoimmune diseases, early joint use to improve disease-modifying antirheumatic drug treatment of RA has reached a consensus, but these joint programs in clinical remission rate was 40% -70%. Condition the development of joint deformities, there is no treatment to repair the cartilage and bone destruction. So finding new and effective treatment of RA is the rheumatism academic circles at home and abroad committed to research hot spot. Mesenchymal stem cells (MSCs) is a high degree of self-proliferation and differentiation potential of stem cells. Recent studies have found that its immunomodulatory effects. Our previous studies have confirmed that MSCs transplantation can improve joint symptoms of collagen-induced arthritis (CIA) in rats. The subject of CIA rats, divided into the early treatment group and the late treatment group by the rat tail vein infusion of MSCs from transplanted rat immune cells and immune molecules, such as multi-angle to explore the mechanism of its immunomodulatory effects; from MSCs in vivo migration, colonization, differentiation, many ways to explore the mechanism of repair of articular cartilage and bone. Experimental and theoretical basis for the clinical use of MSCs transplantation in the treatment of RA.

英文关键词： Mesenchymal stem cells；Rheumatoid arthritis；Green fluorescent protein；Immunoregulation；Differentiation