集成电化学方法的宽频自适应差分石英晶体微天平的研制及其在肿瘤标志物检测中的应用

项目名称： 集成电化学方法的宽频自适应差分石英晶体微天平的研制及其在肿瘤标志物检测中的应用

项目编号： No.21227008

项目类型： 专项基金项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 张书圣

作者单位： 临沂大学

项目金额： 310万元

中文摘要： 本项目拟设计开发集成电化学方法的宽频自适应差分石英晶体微天平系统，针对电极修饰、生物分子识别与生物化学循环放大反应等应用的需求，引入宽频自适应响应系统；针对多种性质的生物分子的广泛检测的需求，将时间分辨的差分测量技术引入到仪器系统设计中，提高测量的精确度；集成电化学测量技术，结合时间分辨测量技术，构建同时适用于稳态电化学方法和暂态电化学方法的电化学-石英晶体微天平联用体系。该系统与传统QCM仪器相比，预计更广泛适用于电化学与石英晶体微天平同时检测的不同种类、不同性质的生物待测分子的分析并将灵敏度从pM级提高到fM级，可应用于肿瘤标志物的检测，拓展了其应用研究领域，并具备较高的检测灵敏度和特异性，从而形成自主知识产权的集成电化学方法的宽频自适应差分石英晶体微天平分析平台。

中文关键词： 石英晶体微天平；肿瘤标志物；信号放大；修饰芯片；

英文摘要： In this study, the electrochemistry-integrated broadband adaptive differential quartz crystal microbalance would be developed. The broadband adaptive response capacity would be introduced to address the demand of electrode modification, biological molecules recognition and biochemical cycling amplification reaction; and the time-resolved differential measurement technique would be introduced to address the demand of the universal measurement of various bioanalytes, to which the analysis sensitivity would be expected to be improved. The electrochemical technique would be integrated into this system together with the time-resolving technique to enable the QCM system to be capable to carry out both the steady-state and the transient electrochemical methods. Compared with the traditional QCM units, the system in this study would be more universally adaptive to the contemporary detection of bioanalytes of various types and of different properties accompanying the superiority of improving the sensitivity from pM to fM, and can be utilized in the detection of tumor markers, broading the application space of QCM technique. Thus, the universal QCM bioanalysis platform with independent intellectual property rights can be established.

英文关键词： quartz crystal microbalance；cancer markers；signal amplification；the chip modification；