项目名称: 共轭聚电解质/石墨烯复合探针的制备及其生物传感性能研究
项目编号: No.21274074
项目类型: 面上项目
立项/批准年度: 2013
项目学科: 数理科学和化学
项目作者: 李春
作者单位: 清华大学
项目金额: 80万元
中文摘要: 共轭聚电解质良好的水溶性、独特的倍增光学响应性使其在生物传感领域具有重要的应用前景。实现倍增光学响应的主要策略之一是构建共轭聚电解质-电子/能量受体离子复合物,通过给受体间的电子或能量转移实现对特定底物的高灵敏度检测。因此,探寻高效的共轭聚电解质-电子/能量转移体系是提高传感性能的一个重要方法。石墨烯是一种新兴的二维碳基纳米材料,具有高效荧光淬灭能力,因此是共轭聚电解质的一个理想复合组分制备高性能复合探针。本项目将设计合成结构可控的水溶性石墨烯,系统地研究其结构与性能关系。发展共轭聚电解质与石墨烯的复合方法,探讨静电、疏水、pi-pi等相互作用及其离子强度对共轭聚电解质与石墨烯相互作用的影响,制备结构可控的共轭聚电解质/石墨烯复合探针。实现对特定生物分子的快速、高选择性、高灵敏度检测与区分。该项目的确立与完成将对共轭聚电解质/石墨烯复合材料的合成及其传感性能研究提供理论基础与技术支撑。
中文关键词: 石墨烯;共轭聚电解质;复合材料;组装;传感器
英文摘要: Conjugated polyelectrolytes (CPEs) provide a unique platform for the development of sensors for biologically relevant targets in view of their water-solubility and signal-amplification effect. One of the main signal transduction mechanisms for amplification effect is to construct CPE-electron/energy acceptor ionic complexes, and the analyte detection with high sensitivity and selectivity can be realized through electron transfer (ET) or fluorescence resonance energy transfer (FRET) between CPE and acceptors. Therefore, exploration of efficient CPE-acceptor ionic complex is a key step towards the construction of biosensors with high sensitivity. Graphene, a single-atom-thick two-dimensional carbon based nanosheet, can induce efficient fluorescence quenching of nearby fluorophores via fluorescence resonance energy transfer (FRET) or nonradiative dipole-dipole coupling. Thus, graphene is an ideal component for preparing high-quality hybrid probe with CPE. In the present project, we'll design and synthesis water-soluble graphene and develop strategies for preparing CPE/graphene composite probes for sensing bioanalytes with high sensitivity and selectivity. The influences of electrostatic, hydrophobic, pi-pi stacking and ion strength on the interaction between CPE and graphene will be explored, and the sensing mechan
英文关键词: graphene;conjugated polyelectrolytes;composite materials;assembly;sensor