一个新的花生磷脂酶D在干旱胁迫响应中的功能与作用机制研究

项目名称： 一个新的花生磷脂酶D在干旱胁迫响应中的功能与作用机制研究

项目编号： No.31201239

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 陈四龙

作者单位： 河北省农林科学院粮油作物研究所

项目金额： 25万元

中文摘要： 磷脂酶D（PLD）是一类重要的跨膜信号转导酶，在植物响应干旱胁迫中有重要作用。不同的PLD干旱应答机制截然不同。前期研究中，我们在花生中发现一个新的PLD基因AhPLDα3，可被干旱胁迫诱导并在干旱下持续稳定表达，推测其在干旱胁迫应答中起重要作用，具体功能尚未见报道。因此，本项目拟探明AhPLDα3的时空表达特性；采用过量表达和RNAi干扰技术获得转基因植物，明确其在干旱胁迫应答中的功能；通过分析在干旱或ABA诱导下，AhPLDα3过量表达和抑制后对气孔、细胞膜及ABA介导的干旱胁迫信号因子的影响，探明其响应干旱胁迫的生理生化机制；通过转基因植物芯片表达谱分析，明确AhPLDα3参与干旱信号转导的途径，解析其响应干旱胁迫的分子机制。本研究将为阐明干旱胁迫信号转导机制提供更系统的理论支持，为分子育种提高作物抗旱性提供理论依据和基因资源。

中文关键词： 干旱响应；花生；磷脂酶D；信号转导；基因功能

英文摘要： Phospholipase D (PLD) is a kind of important enzymes in signal transduction of biological membrane and plays a crucial role in responding to drought stress in plant. Some evidences demonstrated the specificity of PLDs in signal transduction with plant species and cellular processes. In our previous studies, a novel PLD gene, AhPLDα3, was isolated from peanut. The gene expression of AhPLDα3 was strongly stimulated by water deficit. More importantly, AhPLDα3 presented a remarkable stability of expression in conditions of progressive drought stress in peanut. Therefore, AhPLDα3 could be greatly important for peanut to respond to drought stress. However, the concrete function of AhPLDα3 has not been reported up to now. In this study, the temporal and spatial expression patterns of AhPLDα3 will be investigated firstly. Furthermore, analyses of plants over-expressing AhPLDα3 gene and silencing endogenous AhPLDα3 by RNAi will provide insights into the function by which this gene is involved in plant drought stress responses. In order to explore the physiological and biochemical mechanisms of AhPLDα3 response to drought, the relationship of stomata, plasma membrane and the essential elements in the signal transduction pathway of ABA induced stomata movement under water stress or treatment of exogenous ABA will be studie

英文关键词： drought stress response；peanut；phospholipase D；signal transduction；gene function