结构与功能生物学研究定时开花基因SOC1在调控竹子开花中的作用

项目名称： 结构与功能生物学研究定时开花基因SOC1在调控竹子开花中的作用

项目编号： No.31270715

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 徐英武

作者单位： 浙江农林大学

项目金额： 82万元

中文摘要： 适时开花是植物遗传繁殖成功的关键，而确定植物何时开花是在外部环境因素影响下由一整套开花基因共同精细调控的的结果。双子叶拟南芥定时开花关键主效基因SOC1 （Suppressor of Overexpression of Constans1）, 控制花发育身份基因LFY的表达，同时其自身表达受上游FLC抑制和CO1 激活。竹子开花具有时间上的不确定性，开花会引起整片竹林的死亡。在宏观上单子叶竹子是通过什么途径诱导开花的远不像模式植物那么清晰，那么在基因水平上竹子开花调控机理与模式植物有何不同？本项目以开花和不开花雷竹为材料，选择与拟南芥SOC1同源的基因为靶标，研究SOC1在雷竹开花中的生物学功能及其表达特征；分析其与上下游主要调控基因的互作关系；结合结构生物学方法，深度研究SOC1蛋白质的三维空间结构，分析结构与功能之间的关系。本研究对深化竹子成花机理，促进竹产业的生产与发展具有重要意义。

中文关键词： SOC1 基因；开花；竹子；基因调控；蛋白表达

英文摘要： Timely flowering is a critical step for plants to success their genetic breeding, flowering time is fine tuned by a set of genes under the influence of environmental factors. SOC1 (Suppressor of Overexpression of Constans1) is a major gene that determines the timing of flowering in dicotyledonous Arabidopsis. SOC1 controls the expression of flower development identity gene LFY, while its own expression is inhibited by upper stream gene FLC (Flowering of Locus C) and activated by CO1. It is uncertain when bamboo flowerings, however flowering causes the death of the whole bamboo forestry. Disregard the fact that it is not so clear what environmental factors induce flowering, on the level of gene regulation, are there any differences between bamboo flowering and model plants? This proposal targets SOC1 gene from bamboo praecox to study its biological functions, its expression characteristics, and its relationship with upstream and downstream genes. SOC1 3D protein complex crystal structures will be determined to analyze the relationship between structure and function. This study deepens our understanding of the regulation mechanism for bamboo flowering, and promotes the production and development for bamboo industry.

英文关键词： SOC1 gene；Flowering；Bamboo；Gene regulation；Protein expression