转录因子FUSCA3在拟南芥三酰甘油积累中的功能解析

项目名称： 转录因子FUSCA3在拟南芥三酰甘油积累中的功能解析

项目编号： No.31270295

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 张猛

作者单位： 西北农林科技大学

项目金额： 78万元

中文摘要： 了解植物三酰甘油合成的调控机理对于油料作物的分子育种和基因工程改良具有极其重要的意义。转录因子FUSCA3（FUS3）是拟南芥拟南芥胚发育的主调控因子之一，在贮藏物质积累中扮演重要的角色，而在三酰甘油积累中的调控地位尚不清楚，还不了解其与WRINKLED1(WRI1)的作用关系。我们在前期研究中首次获得了FUS3过量表达能够激活三酰甘油积累的直接确凿的证据。本研究拟以此发现为出发点，从两个方面对FUS3在三酰甘油复杂的调控网络中的作用机理进行剖析：一是通过分析wri1*FUS3和WRI1*fus3的含油量变化、分析FUS3与WRI1的表达模式以及FUS3蛋白与WRI1启动子的结合，研究WRI1在FUS3调节三酰甘油积累中的作用；二是通过全基因组DNA结合位点分析、转录组分析、生物功能分析、FUS3蛋白与靶DNA体外结合、靶基因表达模式等研究，确定FUS3与三酰甘油积累有关的下游基因。

中文关键词： 转录因子；FUSCA3；三酰甘油；调控因子；调节机理

英文摘要： Triacylglycerol (TAG) is a main component of plant oil, which is substrate and energy source for seedling development of next generation. Understanding regulation mechanism of TAG biosynthesis is of great significance to plant developmental theory and bio-engineering of oil crops. FUSCA3 (FUS3) is a transcription factor of B3 family. As one of master regulators in Arabidopsis embryo development, FUS3 plays very important roles in accumulation of storage materials. Our latest results firstly show that FUS3 triggers TAG accumulation in vegetative cells or tissues, which provides new direct evidence to prove its role in oil accumulation. WRINKLED1 (WRI1) is an AP2 transcription factor, which is proved to be a more specific regulator of oil accumulation. Intensive studies provide insight into the regulation network of embryo-related transcription factors. FUS3 and WRI1 are proposed to be downstream regulators of LEC1 and LEC2 in the regulation network of oil accumulation. How does FUS3 regulate TAG biosynthesis? Is this regulation WRI1-dependent? They are still open questions we need to answer. Based on literature search and our transcriptome analysis of FUS3 over-expression seedlings, WRI1 is presume to be a downstream regulator of FUS3 in the process of oil accumulation. We hereby proposed a hypothesis that FUS3 a

英文关键词： Transcription factor；FUSCA3；triacylglycerol；regulator；regulation mechanism