转录因子NbNAC1调控植物系统性抗性防御病毒入侵的作用研究

项目名称： 转录因子NbNAC1调控植物系统性抗性防御病毒入侵的作用研究

项目编号： No.31500209

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 朱峰

作者单位： 扬州大学

项目金额： 20万元

中文摘要： NAC转录因子在调控植物应答生物胁迫起着重要的作用，然而NAC 转录因子调控植物激素信号传导途径来应答病毒入侵的分子机制还很不清楚。前期我们的研究揭示了一个全新的水杨酸(SA)和茉莉酸(JA)相互作用机制调控系统性抗性应答病毒入侵。当植物遭受病毒的入侵，植物首先诱导JA信号来建立早期的系统性防御反应，而SA信号则在病毒接种后期被激活。我们将通过构建NbNAC1过表达和抑制植物来探究NbNAC1调控SA和JA介导的系统性抗性应答病毒入侵的分子机制。我们将开展以下研究：1) 过表达或抑制NbNAC1的表达对植物系统性抗性以及对SA和JA信号途径的影响；2) 同时缺乏激素信号与NAC信号对植物系统性抗性的影响；3) NbNAC1调控SA和JA合成及它们下游相关防御基因的表达。该研究将为利用基因工程原理改良农作物的抗逆性，培育优良品种，增强植物对病原菌的抵抗力提供重要的理论依据。

中文关键词： 抗病反应；NbNAC1；转录因子；；系统性抗性；水杨酸和茉莉酸；烟草花叶病毒

英文摘要： It is well established that NAC transcription factors play essential roles in response to biotic stress. However, the molecular mechanism how NAC transcription factors regulate plant hormones signal transduction pathway in response to virus infection is unknown. Previous studies revealed a new mechanism for the interaction between JA and SA in mediating systemic resistance response to virus infection. When the plants are subjected to virus invasion, they will trigger JA signaling immediately for establishing early initiation phase of systemic resistance. After that, SA signaling is activated strongly to establish a broad systemic resistance. We will use NbNAC1 overexpression and knockdown plants to study the molecular mechanism for NbNAC1 regulation the SA and JA in mediating systemic resistance response to virus invasion. We will carry out the below studies: 1) the effect of overexpression or knockdown NbNAC1 on plant systemic resistance and SA and JA signal pathways; 2) the effect of knockdown plant hormone and NAC signal on plant systemic resistance; 3) How the NbNAC1 regulates SA and JA synthesis and the expression of their downstream related defense genes. Our studies will provide a theoretical foundation for using genetic engineering to improve crop resistance, cultivating good varieties and enhancing the plants’ resistance to pathogens.

英文关键词： Resistance response;NbNAC1 transcription factor;Systemic resistance;Salicylic acid and jasmonic acid;Tobacco mosaic virus (TMV)