白头翁汤调控Rho/ROCK信号通路治疗放射性肠炎的机制研究

项目名称： 白头翁汤调控Rho/ROCK信号通路治疗放射性肠炎的机制研究

项目编号： No.81473683

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 陆金根

作者单位： 上海中医药大学

项目金额： 75万元

中文摘要： 放射性肠炎(RE)是盆腔、腹腔、腹膜后恶性肿瘤经放射治疗引起的肠道并发症。晚期肠壁引起纤维化，肠腔狭窄或穿孔，腹腔内形成脓肿、瘘道和肠粘连等严重影响患者的生存质量。放射线在中医属火热毒邪，RE病机为射线之邪毒侵袭，热毒炽盛，灼伤经络，热毒深陷血分，下迫大肠。而出自《伤寒论》的经典名方白头翁汤,以清热解毒，凉血止痢为治则，大量临床实验已证明其治疗本病疗效确切，但对其抗纤维化的机制尚未见研究报道。本课题拟构建大鼠放射性肠炎纤维化模型，筛选出该模型中的致纤维化相关因子，于在体和离体具体研究白头翁汤在RE纤维化发生、发展不同时期的作用机制，并进一步探讨白头翁汤是否对Rho/ROCK 通路发挥作用，从而研究白头翁汤对肿瘤患者盆腔放疗后直肠纤维化的潜在保护作用，为其应用于放射防护提供理论基础。

中文关键词： 放射性肠炎；Rho/ROCK信号通路；白头翁汤；纤维化

英文摘要： Radiation enteritis is an inrestinal complication after radiotherapy beacause of abdominal, pelvic retroperitoneal malignant tumor. There are fibrosis, luminal stenosis or perforation, abscess, fistula and intra-abdominal intestinal adhesion formation in the advanced stages of the disease, severely affect the quality of life of patients. Radiation in Chinese medicine is a heat toxin , it's pathogenesis is ray of toxin invasion, toxic heat flourishing.Pulsatilla decoction from treatise on Febrile Diseases ,with heat-clearing and detoxicating, cooling blood to stop diarrhea treatment, a large number of clinical experiments have proved that the treatment of this disease is exact, but the mechanism of its anti fibrosis are not reported. This project aims to construct radioactivity enteritis fibrosis model rats, selected in the model induced fibrosis related factor; in the mechanism of action in vivo and in vitro study of Pulsatilla decoction, specific occurred in the development of fibrosis in different periods; and further to explore whether Pulsatilla Decoction on Rho/ ROCK pathway play a role. In order to study the potential protective effects of pelvic tumor after radiotherapy in patients with rectal fibrosis, for its application to provide a theoretical basis for radiation protection.

英文关键词： radiation enteritis;Rho/ROCK signaling;Pulsatilla Decoction;fibrosis