药蒲公英诱导HO-1活性成分及其抗阿尔茨海默病的多途径机制研究

项目名称： 药蒲公英诱导HO-1活性成分及其抗阿尔茨海默病的多途径机制研究

项目编号： No.31500288

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 李斌

作者单位： 青岛科技大学

项目金额： 20万元

中文摘要： 阿尔茨海默病 (AD) 作为老年性痴呆的主要类型，其发病数量逐年上升，在发达国家已成为继心脏病、癌症和中风之后第四位的死亡原因。药蒲公英作为传统医药，具有多靶点、副作用小等特点，在防治各类疾病中表现出单靶点治疗的西药所不具备的优势。课题组在前期研究中发现，药蒲公英提取物具有抑制小鼠小胶质细胞NO生成，减少谷氨酸引起的小鼠海马细胞毒性等活性，表现出了一定的改善AD潜力，说明药蒲公英的多靶点性。另外，药蒲公英提取物在小胶质细胞、海马细胞，均诱导HO-1mRNA的表达。就目前为止国内外关于药蒲公英诱导HO-1、预防和治疗AD的作用和机制方面的研究尚未见报道。基于中药、天然药物多成分与AD疾病多靶点、多途径的相关性研究，课题组拟从细胞水平评价、探讨药蒲公英活性部位或活性化合物预防和治疗AD活性，阐明其作用机制，利用AD动物模型，再评价和验证其活性，为作用物质基础及作用机制研究提供一种探索思路。

中文关键词： 阿尔茨海默病；药蒲公英；小胶质细胞；海马细胞；HO-1

英文摘要： Alzheimer’s disease is the leading cause of dementia in the elderly and is the fourth leading cause of death in developed nations. Oxidative stress and neuroinflammation were implicated in many neurodegenerative diseases, including Alzheimer’s disease. Taraxacum officinale has long been used in herbal medicine for their choleretic, antiheumatic and diuretic properties. The present study proposed to examine the role of Taraxacum officinale as an anti-inflammatory and anti-oxidative heme oxygenase-1 (HO-1) inducer in BV2 microglia cells and mouse hippocampal HT22 cells. The effect of Taraxacum officinale on the ntric oxide production was analyzed by NO assay and the effect of cell viability was determined by MTT assay. In addition, as a part of ongoing research to identify natural products that can induce HO-1 expression in vitro, we have shown here that the ethanol extract of Taraxacum officinale was shown to significantly increase the levels of HO-1 in both BV2 microglia and HT22 cells. These results suggest that Taraxacum officinale possesses therapeutic potentials against Alzheimer’s disease that are induced by oxidative stress and neuroinflammation. Although several beneficial effects of Taraxacum officinale have been investigated, its direct molecular targets and the mechanism of action on the protection/defense system against Alzheimer’s disease has not been reported yet.

英文关键词： Alzheimer’s disease ;Taraxacum officinale ;microglia cells ;hippocampal cells;HO-1