致病相关分泌蛋白BC1G_08363和BC1G_15201在灰葡萄孢致病过程中的作用及其机理研究

项目名称： 致病相关分泌蛋白BC1G_08363和BC1G_15201在灰葡萄孢致病过程中的作用及其机理研究

项目编号： No.31501587

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 朱闻君

作者单位： 武汉轻工大学

项目金额： 19万元

中文摘要： 由灰葡萄孢引起的灰霉病可造成农作物严重减产，而致病相关分泌蛋白在灰葡萄孢的侵染过程中起着重要作用，抑制其致病相关分泌蛋白的活性将会有效防控灰霉病害。但迄今为止，对抑制植物抗性或诱导植物死亡的灰葡萄孢致病相关分泌蛋白的功能机理的研究还相对较少。在前期研究中，通过对灰葡萄孢侵染早期的蛋白质分泌组的质谱分析，筛选到两个富含半胱氨酸的分泌蛋白BC1G_08363和BC1G_15201，其基因敲除转化子的致病力降低，初步表明这两个分泌蛋白是致病相关蛋白。本研究拟从这两个基因及其敲除转化子着手，通过基因互补和超表达技术进一步明确这两个基因与致病力的关系，并通过基因表达分析、蛋白定位、蛋白活性检测及互作蛋白的筛选等方法深入研究这两个基因的功能及其调控灰葡萄孢致病的作用机制，研究结果将进一步阐明灰葡萄孢的致病机理，为其致病相关分泌蛋白阻断策略的研发、抗病作物的培育和新型杀菌剂的研制提供新的线索和理论依据。

中文关键词： 灰葡萄孢；灰霉病；致病相关分泌蛋白；致病机理；富含半胱氨酸的分泌蛋白

英文摘要： The necrotrophic fungal phytopathogen Botrytis cinerea could cause gray mold disease in crops, causing serious economic losses. The pathogenicity-associated secretory proteins play significant roles during the infection of B. cinerea on the host plants. The crop gray mold disease would be controlled effectively and safely by inhibiting the activity of B. cinerea pathogenicity-associated secretory proteins. However, until now, the genes screen and the functional mechanisms of the pathogenicity-associated secretory proteins involved in interference of plant defense or inducing necrosis have hardly been investigated. In our previous study, the B. cinerea secretome during the early stage of infection was analyzed by LC-MS/MS and two cysteine-rich secretory proteins (BC1G_08363 and BC1G_15201) were screened. Targeted deletion of BC1G_08363 and BC1G_15201 respectively resulted in a significant reduction in virulence, indicating that BC1G_08363 and BC1G_15201 are pathogenicity-associated secretory proteins. In this study, the correlation between the two genes and the B. cinerea pathogenicity will be defined further by the gene complementation and over expression assaying. And also, the function mechanisms of the two genes in B. cinerea infection will be further characterized by the gene expression analysis, protein location, activity analysis and the yeast two-hybrid system. The results of this study will be useful in elucidating B. cinerea pathogenesis and also provide the new theoretical basis for the exploration of inhibition strategy on the activity of pathogenicity-associated secretory proteins, cultivation of the gray mold-resistant crops and the development of new type fungicide.

英文关键词： Botrytis cinerea;gray mold;pathogenicity-associated secretory proteins;pathogenesis; cysteine-rich secretory proteins