基于微生物群落结构解析不同C/N对雌激素去除的影响研究

项目名称： 基于微生物群落结构解析不同C/N对雌激素去除的影响研究

项目编号： No.51508287

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 其他

项目作者： 陈栋

作者单位： 青岛理工大学

项目金额： 20万元

中文摘要： 水环境中以ng/L水平存在的雌激素，是引起水生生物雌性化的主要原因物质，污水处理厂中的雌激素能否被有效降解成为亟需解决的科学问题。在实际污水处理过程中，进水水质的C/N不仅会影响COD、N、P的去除，而且会通过影响活性污泥中异养菌和AOB的群落结构而影响雌激素的去除。因此，本项目通过SBR系统处理不同C/N人工配水，测定对COD和N的去除率，并利用ARISA、TRFLP、FISH和克隆文库等分子生态学技术解析活性污泥系统中异养菌和AOB的群落结构变化。待SBR系统运行稳定后，分别投加E2和EE2，利用GC-MS方法测定两种雌激素的去除率。此外，取SBR系统运行稳定后的活性污泥，进行静态实验，探讨不同SBR活性污泥系统对雌激素吸附性能差异；并通过添加ATU抑制硝化作用，进而确定异养菌和AOB在降解雌激素上各自的作用。研究结果为污水处理厂深化脱氮除磷的同时，提高雌激素的去除效率提供理论指导。

中文关键词： 微生物群落结构；C/N；；雌激素

英文摘要： Steroid estrogen, existed in ng/L levels in water environment, is the main substance causing the feminization of aquatic organisms. In the sewage treatment plant, whether the estrogen could be effectively removed is becoming a scientific problem to be resolved urgently. In the actual process of sewage treatment, the C/N of wastewater could not only influence the removal of COD、N、P and other nutrients, but also affect the removal of estrogen through changing the microbial community structure of heterotrophic and AOB in the activated sludge. Therefore, this study deals with different C/N artificial wastewater by SBR system, and determines the removal efficiency of COD and N, then analyzes the microbial community structure of heterotrophic and AOB in the activated sludge using molecular ecology technology, such as ARISA、TRFLP、FISH and clone library. After the SBR system runs stably, add the E2 and EE2, and examine their removal efficiency by GC-MS. Furthermore, we take the activated sludge after the system runs stably, and conduct static experiments. On the one hand, adsorption performance of different SBR activated sludge system will be discussed. On the other hand, what role of heterotrophic bacteria and AOB paly in the degradation of estrogen will be further studied through adding ATU to inhibit the nitrification. The results will provide the theory guide to improve the removal efficiency of estrogen for sewage treatment plant when deepen the removal of nitrogen and phosphorus.

英文关键词： microbial community ;C/N ;estrogen