可见光和UV-B协同诱导梨果皮花青苷合成的光信号应答分子机制研究

项目名称： 可见光和UV-B协同诱导梨果皮花青苷合成的光信号应答分子机制研究

项目编号： No.31471852

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 滕元文

作者单位： 浙江大学

项目金额： 87万元

中文摘要： 红梨着色依赖果皮内花青苷的积累，需要高强度的光照，我们前期的研究表明可见光和UV-B可协同诱导红梨果皮花青苷的积累。目前针对红梨着色分子机制的研究多集中于花青苷合成相关的调节基因和结构基因，而对上游光信号转导路径关注较少。光信号作用于植物光受体，并通过信号转导途径向下游传递调控花青苷合成，相关机理已在模式植物上阐明，但还没有理论可以解释可见光和UV-B协同促进红梨着色的现象。本研究拟以具有不同着色模式的红梨品种为试材，通过调节可见光和UV-B来诱导红梨差异着色，通过生理指标测定、关键光信号路径基因克隆、基因表达水平检测、基因之间的互作模式探索和相关蛋白丰度检验等方面探索可见光和UV-B协同诱导红梨花青苷合成的分子机理。研究结果将有助于阐明红梨花青苷合成的光信号应答分子机制，明确关键调控位点，丰富花青苷合成的生物学理论，为利用分子技术手段培育红梨新品种和开发着色调控技术提供理论支撑。

中文关键词： 红梨；花青苷；光信号；协同效应；分子机制

英文摘要： Red coloration on in pear peel is known to depends on the accumulation of anthocyanin, and it requries high-intensity irradiation.Our prelimineray study showed that visible light and UV-B synergistically induced anthocyanin accumulation in red pear. Current researches on the molecular mechanism of red pear coloration mainly focused on regulatory genes and structual genes involved in anthocyanin biosynthesis, but less attention has paid on the upstream light signal transduction. Plant photoreceptors receive light signal, and transfer the signal to regulate the biosynthesis of downstream procedures including anthocyanin, molecular basis of light mediating anthocyanin accumulation has been clarified in model plant, but mechanism of visible light and UV-B synergisitically inducing pear coloration has not known yet. In this project, typical red pears including red Chinese sand pear and European pear with different coloration patterns will be used to study molecular mechanism of coloration in red pears in response to light signal. Visible light and UV-B will be used to induce anthocyanin accumulation in red pears. Key candidate genes participating in light signal transduction pathway related to anthocyanin biosynthesis will be cloned, level of physiological indicators, gene expression and protein abundance will be determined, and interaction pattern of key genes will be investigated. The research will be helpful to clarify the light regulation mechanism of anthocyanin biosynthesis in red pears, to determine the key regulatory sites, which will enrich the theory of molecular mechanism of plant anthocyanin biosynthesis and provide thoretical basis for breeding new red pear cultivars by molecular methods and develop technology for good coloring regulation.

英文关键词： red pear;anthocyanin;light signal;synergism;molecular mechanism