桦木酸对氧化应激诱导淋巴细胞凋亡的作用及其调控机理

项目名称： 桦木酸对氧化应激诱导淋巴细胞凋亡的作用及其调控机理

项目编号： No.31201964

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 畜牧学与草地科学、兽医学、水产学

项目作者： 易金娥

作者单位： 湖南农业大学

项目金额： 23万元

中文摘要： 桦木酸属于植物源性五环三萜类物质，具有提高免疫力，抗氧化应激和抗肿瘤等多种生物学活性，作为免疫调节剂和抗氧化剂在动物养殖方面具有广阔的应用前景，但其作用分子机制还不清楚。本项目提出桦木酸的免疫调节作用有赖于抗氧化应激机能的发挥这一假设，以小鼠淋巴细胞为研究模型，应用细胞生物学与分子生物学的实验方法，在氧化应激状态下，研究桦木酸对淋巴细胞氧化还原系统与抗氧化酶系的影响；细胞凋亡与线粒体功能的影响；Caspase和ASK1/JNK 信号通路中凋亡调节蛋白活性与基因表达量的影响，以及探索桦木酸对Bcl-2、Bax、Caspase-9、-3 、ASK1、JNK和P38 等蛋白质磷酸化水平的变化与信号转导通路的调控；从基因表达水平和信号转导通路等角度揭示桦木酸对氧化应激诱导淋巴细胞凋亡的作用与调控机理。本项目将为桦木酸的临床应用提供科学依据，对促进动物健康养殖和提高畜产品质量都有重要意义。

中文关键词： 桦木酸；淋巴细胞；细胞凋亡；氧化应激；线粒体

英文摘要： Betulinic acid is a naturally occurring pentacyclic triterpene that exhibits a variety of biological activities，including immunomodulatory, antioxidation, anti-tumor, and so on. Notably, betulinic acid may have promising clinical applications in the animal farming as an immunomodulator and antioxidant. However, the molecular mechanism of betulinic acid exerts its physiological influences still remains unclear. The project proposes the hypothesis that betulinic acid modulates the immune functions by regulating their redox environment and its related sigaling pathways. In this project, mouse lymphocytes will be used as a model to explore the roles and mechanism of betulinic acid on cell apoptosis induced by oxidant stress, using the approaches of celluar biology and molecular biology. The redox system and antioxidase activity, cellular apoptosis and mitochondrial function of lymphocyte induced by oxidant stress will be determined. The effect of betulinic acid on genes expression, regulatory protein activity and protein phosphorylation in the signal transduction pathway of Caspase and ASK1 / JNK, including Bax, Bcl-2, as well as caspase-9,-3, ASK1, JNK, and p38, will be measured. This project would facilitate better understanding the function and molecular mechanisms of betulinic acid on lymphocyte apoptosis induce

英文关键词： Betulinic acid；lymphocyte；apoptosis；oxidative stress；mitochondria