小麦TaZFP15调控植株应答低磷分子网络和生化途径研究

项目名称： 小麦TaZFP15调控植株应答低磷分子网络和生化途径研究

项目编号： No.31201674

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 李小娟

作者单位： 河北农业大学

项目金额： 23万元

中文摘要： 植株体内存在复杂且精细的应答低磷逆境转导通路，特定转录因子作为通路重要成员在调控下游低磷应答基因的表达中发挥重要作用。申请者前期研究表明，小麦C2H2型锌指蛋白类（ZFP）转录因子基因TaZFP15对低磷逆境产生明显应答，在增强植株抵御低磷胁迫中发挥着重要功能。本项研究拟在前期工作基础上，进一步鉴定TaZFP15的亚细胞定位特征，通过原位杂交和启动子不同长度片段驱动报告基因（GUS）表达研究，揭示TaZFP15应答低磷的转录调控机制。以目前已获得的超表达TaZFP15的转基因植株为材料，利用基因芯片技术、蛋白质组学技术和代谢谱分析技术，系统鉴定TaZFP15调控的下游基因、蛋白和代谢物，解析该小麦转录因子调控的分子网络，阐明其调控植株应答和抵御低磷逆境的生化代谢途径。本项研究将从转录调控角度，深刻揭示小麦应答和抵御低磷逆境的分子机理，为今后创制磷高效小麦及其他作物种质（品种）提供理论依据。

中文关键词： 小麦；ZFP转录因子；低磷逆境；基因功能；分子机理

英文摘要： As important members in the low-Pi stress signaling transduction system, distinct transcription factors play critical roles in it by fine-regulating the expression of the downstream genes. Our previous results clearly indicated that TaZFP15, a zinc-finger type transcription factor gene in wheat, is involved in responding to low-Pi stress and up-regulation of this transcription factor gene could significantly improve the capacities of plant growth, phosphorus acquisition, and phosphorus utilization under deficient-Pi condition. In this study, we will further identify the subcellular location of TaZFP15 based on in situ hybridization and clarify the transcriptional regulation mechanism regulated by TaZFP15 via analyzing expression of fusions constructed with truncated promoter fragments and reporter gene GUS. Using the T3 transgenic wheat lines we have generated, we will systematically identify the differentially expressed genes, proteins, and metabolic related substances that regulated by TaZFP15 based on techniques of gene chip and hybridization, protein 2-DE isolation and corresponding MS identification, and LS-MS and GS-MS, respectively. Based on above research, we will dissect the molecular network regulated by TaZFP15 and systematically determine the biochemical metabolic pathway to respond and withstand the

英文关键词： Wheat；ZFP type transcription factor；Pi deprivation；Gene function；Molecular mechanism