基于微流控芯片的病毒细胞间传播行为研究

项目名称： 基于微流控芯片的病毒细胞间传播行为研究

项目编号： No.21475099

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 张志凌

作者单位： 武汉大学

项目金额： 90万元

中文摘要： 病毒细胞间传播是一种重要的病毒传播方式，与病毒逃逸宿主免疫系统以及药物治疗有极大关系，深入认识病毒细胞间传播机制有助于新型抗病毒药物设计和病毒相关疾病治疗方案的确定。病毒的侵染和传播行为与宿主细胞骨架结构密切相关，而细胞骨架排布又受到细胞附着基底物理形貌及结构的影响，与细胞骨架结构相关的病毒侵染和传播过程很可能也会受到基底形貌及结构的影响。微流控技术可实现集成化的细胞分析，实时监控细胞生长、增殖、迁移及凋亡等细胞行为，在细胞-细胞以及细胞-细胞外微环境相互作用研究方面体现出了巨大优势和重要作用，已成为（单）细胞分析的重要平台技术。本项目旨在于利用微流控芯片在细胞外微环境调控上的优势，研究微环境（主要是基底表面结构）对病毒细胞间传播的影响，建立研究基底结构对病毒细胞间传播影响的模型和方法，初步揭示病毒在细胞间各向异性传播机制，推动病毒与宿主细胞相互作用研究新型平台技术的建立。

中文关键词： 微流控；病毒；细胞间传播；表面结构

英文摘要： As an important virus transmission mode, cell-to-cell spread could help virus escape immune system and is resistant to antivirus drugs. Deep understanding of virus cell-to-cell spread mechanism may contribute to the development of novel antivirus drug and the therapy for infectious diseases. Cytoskeleton is invovled in virus infection and transmission. Reorganization and reconfiguration of cytoskeleton is affected by the morphology and surface structure of substrate for cell adhersion, which has the potentail to affect the cytoseleton related virus infection and transmission. Microfluidic chip has been used for the integrated cell analysis to monitor various cell behaviours, including cell growth, migration, apoptosis and so on, which has shown great advantages and potentail on cell-cell and cell-extracellular microenvironment interactions. Microfluidics has become an important platform technology for cell or singel cell analysis. The aim of this project is to study the influence of substrate surface structure on virus cell-to-cell spread under controlled extracellular mircoenvironment on microfluidic chips, and to develop the model and method for study on interaction between substrate sruface structure and cell-to-cell spread. The study will help to unravel the mechanism of anisotropic cell-to-cell spread, and establish a novle platform technology for the study of virus-host cell interaction.

英文关键词： Microfluidic;Virus;Cell to cell spread;Surface structure